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PPAR gamma Recombinant Rabbit Monoclonal Antibody [JF101-4]

Usd: 385

Specification

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Catalog# ET1702-57

PPAR gamma Recombinant Rabbit Monoclonal Antibody [JF101-4]

Application

  • WB

Reactivity

  • Human

  • Rat

Predicted reactivity

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PPAR gamma Recombinant Rabbit Monoclonal Antibody [JF101-4]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PPAR gamma aa 107-150 / 505.

Species Reactivity

Human, Rat (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB

Target Molecular Weight

Predicted band size: 58 kDa

Positive Control

PC-12 cell lysate, K562 cell lysate.

Conjugation

unconjugated

Clone Number

JF101-4

RRID

AB_3070319

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARγ is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPARγ activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPARγ may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPARγ2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARγ2 gene maybe correlated with abdominal obesity in type 2 diabetes.

Background References

1. Yu P et al. Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine. PLoS One 11:e0154949 (2016).

2. Yu YH et al. PKC-ALDH2 Pathway Plays a Novel Role in Adipocyte Differentiation. PLoS One 11:e0161993 (2016).

Sequence Similarity

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Tissue Specificity

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

Post-translational Modification

O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.; Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

P37231

Synonyms

CIMT1 antibody

GLM1 antibody

NR1C3 antibody

Nuclear receptor subfamily 1 group C member 3 antibody

OTTHUMP00000185032 antibody

OTTHUMP00000185036 antibody

Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody

Peroxisome proliferator activated receptor gamma 1 antibody

Peroxisome Proliferator Activated Receptor gamma antibody

Peroxisome proliferator-activated receptor gamma antibody

Expand

Images

  • Western blot analysis of PPAR gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-57" style="font-weight: bold;text-decoration: underline;">ET1702-57</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: PC-12 cell lysate<br />Lane 2: K562 cell lysate

    Western blot analysis of PPAR gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: PC-12 cell lysate
    Lane 2: K562 cell lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation