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Midkine Recombinant Rabbit Monoclonal Antibody [JF096-5]

Usd: 385

Specification

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Catalog# ET1702-64

Midkine Recombinant Rabbit Monoclonal Antibody [JF096-5]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Midkine Recombinant Rabbit Monoclonal Antibody [JF096-5]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Midkine aa 100-143 / 143.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 16 kDa

Positive Control

HepG2 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, human liver tissue, human pancreas tissue.

Conjugation

unconjugated

Clone Number

JF096-5

RRID

AB_3070326

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Midkine, or MK, is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis. MK expression is tightly regulated during embryonic development by steroid receptors of the retinoic acid superfamily. The mature human MK protein is 118 amino acids in length and contains five intrachain disulfide bonds. MK is a non-glycosylated protein that shows greater than 87% identity between human and mouse. The carboxy-terminus of MK contains the principle heparin-binding site and the molecule’s neurite-promoting sequences; both the amino- and carboxy-terminal sequences are required for the molecule’s neurotrophic properties. An association between overexpression of MK and colon adenocarcinoma has been shown in families suffering from familial polyposis. In addition, MK functions to enhance the activity of plasminogen activator (PA).

Background References

1. Yamashita T et al. Serum midkine as a biomarker for malignancy, prognosis, and chemosensitivity in head and neck squamous cell carcinoma. Cancer Med 5:415-25 (2016).

2. Yuan K et al. MDK Protein Overexpression Correlates with the Malignant Status and Prognosis of Non-small Cell Lung Cancer. Arch Med Res 46:635-41 (2015).

Sequence Similarity

Belongs to the pleiotrophin family.

Tissue Specificity

Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P21741 Human

Synonyms

Amphiregulin associated protein antibody

Amphiregulin-associated protein antibody

ARAP antibody

FLJ27379 antibody

Mdk antibody

Midgestation and kidney protein antibody

Midkine antibody

MK 1 antibody

MK antibody

MK_HUMAN antibody

Expand

Images

  • Western blot analysis of Midkine on different lysates with Rabbit anti-Midkine antibody (<a href="/products/ET1702-64" style="font-weight: bold;text-decoration: underline;">ET1702-64</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: SH-SY5Y cell lysate<br />Lane 3: U-2 OS cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-64" style="font-weight: bold;text-decoration: underline;">ET1702-64</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Midkine on different lysates with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: U-2 OS cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Midkine antibody (<a href="/products/ET1702-64" style="font-weight: bold;text-decoration: underline;">ET1702-64</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-64" style="font-weight: bold;text-decoration: underline;">ET1702-64</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Midkine antibody (<a href="/products/ET1702-64" style="font-weight: bold;text-decoration: underline;">ET1702-64</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-64" style="font-weight: bold;text-decoration: underline;">ET1702-64</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"