ASPP2 Recombinant Rabbit Monoclonal Antibody [JF09-98]
Catalog# ET1702-79
ASPP2 Recombinant Rabbit Monoclonal Antibody [JF09-98]
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WB
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IF-Tissue
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
ASPP2 Recombinant Rabbit Monoclonal Antibody [JF09-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within N-terminal human ASPP2.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Tissue, IHC-P
Molecular Weight
150 kDa
Positive Control
Hela cell lysate, MCF-7 cell lysate, human kidney tissue, human prostate tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
JF09-98
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:100-1:500
Target
Function
The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.
Background References
1. Song Q., et al. 2016. miR-548d-3p/TP53BP2 axis regulates the proliferation and apoptosis of breast cancer cells. Cancer Med 5:315-324.
2. Gupta G.D., et al. 2015. A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 163:1484-1499.
Sequence Similarity
Belongs to the ASPP family.
Tissue Specificity
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Subcellular Location
Nucleus, perinuclear region.
Synonyms
53BP2 antibody
Apoptosis stimulating of p53 protein 2 antibody
Apoptosis stimulating protein of p53 2 antibody
Apoptosis-stimulating of p53 protein 2 antibody
ASPP2 antibody
ASPP2_HUMAN antibody
BBP antibody
Bcl2 binding protein antibody
Bcl2-binding protein antibody
NY REN 51 antigen antibody
ExpandImages
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Western blot analysis of ASPP2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASPP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-79, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ASPP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-79, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASPP2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-79, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"