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PGP9.5 Recombinant Rabbit Monoclonal Antibody [JM10-59]

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Specification

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Catalog# ET1703-22

PGP9.5 Recombinant Rabbit Monoclonal Antibody [JM10-59]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

  • FC

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PGP9.5 Recombinant Rabbit Monoclonal Antibody [JM10-59]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PGP95 aa 191-223 / 223.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, IHC-Fr

Molecular Weight

Predicted band size: 25 kDa

Positive Control

A-172 cell lysate, SHG-44 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate, A549 cell lysate, 293T cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, N2A, PC-12, mouse spinal cord tissue, mouse skin tissue, mouse brain tissue, mouse colon tissue, mouse cerebrum tissue.

Conjugation

unconjugated

Clone Number

JM10-59

RRID

AB_3070379

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:1,000

  • IHC-P

  • 1:1,000-1:5,000

  • IP

  • Use at an assay dependent concentration.

  • FC

  • 1:1,000

  • IHC-Fr

  • 1:200-1:500

Target

Function

Ubiquitin carboxy-terminal hydrolase L1 (EC 3.1.2.15, ubiquitin C-terminal hydrolase, UCH-L1) is a deubiquitinating enzyme. UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. The catalytic triad of UCH-L1 contains a cysteine at position 90, an aspartate at position 176, and a histidine at position 161 that are responsible for its hydrolase activity.

Background References

1. Takahashi H et al. A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors. J Neurosci 36:8210-27 (2016).

2. Feng W et al.Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells. Asian-Australas J Anim Sci 29:1407-15 (2016).

Sequence Similarity

Belongs to the peptidase C12 family.

Tissue Specificity

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Post-translational Modification

O-glycosylated.

Subcellular Location

Cytoplasm, Endoplasmic reticulum membrane.

UNIPROT

SwissProt: P09936 Human

SwissProt: Q9R0P9 Mouse

SwissProt: Q00981 Rat

Synonyms

Epididymis luminal protein 117 antibody

Epididymis secretory protein Li 53 antibody

HEL 117 antibody

HEL S 53 antibody

NDGOA antibody

Neuron cytoplasmic protein 9.5 antibody

OTTHUMP00000218137 antibody

OTTHUMP00000218139 antibody

OTTHUMP00000218140 antibody

OTTHUMP00000218141 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/2,000 dilution.<br /><br />Lane 1: A-172 cell lysate (20 µg/Lane)<br />Lane 2: SHG-44 cell lysate (20 µg/Lane)<br />Lane 3: U-87 MG cell lysate (20 µg/Lane)<br />Lane 4: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 5: NCI-H1299 cell lysate (20 µg/Lane)<br />Lane 6: A549 cell lysate (20 µg/Lane)<br />Lane 7: 293T cell lysate (20 µg/Lane)<br />Lane 8: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 9: C6 cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br />Lane 11: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 12: Rat brain tissue lysate (30 µg/Lane)<br />Lane 13: LNCaP cell lysate (negative) (20 µg/Lane)<br />Lane 14: K-562 cell lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 7 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/2,000 dilution.

    Lane 1: A-172 cell lysate (20 µg/Lane)
    Lane 2: SHG-44 cell lysate (20 µg/Lane)
    Lane 3: U-87 MG cell lysate (20 µg/Lane)
    Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 5: NCI-H1299 cell lysate (20 µg/Lane)
    Lane 6: A549 cell lysate (20 µg/Lane)
    Lane 7: 293T cell lysate (20 µg/Lane)
    Lane 8: Neuro-2a cell lysate (20 µg/Lane)
    Lane 9: C6 cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)
    Lane 11: Mouse brain tissue lysate (30 µg/Lane)
    Lane 12: Rat brain tissue lysate (30 µg/Lane)
    Lane 13: LNCaP cell lysate (negative) (20 µg/Lane)
    Lane 14: K-562 cell lysate (negative) (20 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 7 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate (10 µg/Lane)<br />Lane 2: A549-si PGP9.5 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/5,000 dilution.

    Lane 1: A549-si NT cell lysate (10 µg/Lane)
    Lane 2: A549-si PGP9.5 cell lysate (10 µg/Lane)

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 3 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-22) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-22) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-22) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-22) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-22) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of SH-SY5Y (positive) and LNCaP (negative) labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of SH-SY5Y (positive) and LNCaP (negative) labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Neuro-2a cells labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of PC-12 cells labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling PGP9.5 with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Pancreas<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Pancreas

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Flow cytometric analysis of SH-SY5Y cells labeling PGP9.5.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1703-22" style="font-weight: bold;text-decoration: underline;">ET1703-22</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SH-SY5Y cells labeling PGP9.5.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1703-22, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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