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GBA Recombinant Rabbit Monoclonal Antibody [JM10-76]

Usd: 385

Specification

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Catalog# ET1703-32

GBA Recombinant Rabbit Monoclonal Antibody [JM10-76]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

GBA Recombinant Rabbit Monoclonal Antibody [JM10-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human GBA aa 477-534 / 536.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 60 kDa

Positive Control

U-87 MG cell lysates, human kidney tissue, human brain tissue, rat brain tissue, mouse pancreas tissue.

Conjugation

unconjugated

Clone Number

JM10-76

RRID

AB_3070389

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:50-1:500

Target

Function

β-Glucosidase is a predominantly liver enzyme which efficiently hydrolyzes β-D-glucoside and β-D-galactoside. Defects in β-glucosidase cause Gaucher disease, an inherited condition distinguished by the accumulation of glucosylceramide within the cells of the reticuloendothelial system. β-Glucosidase is used in enzyme replacement treatment aimed at treating Gaucher disease. The absorption of dietary flavonoid glycosides in humans involves a critical deglycosylation step that is mediated by epithelial β-glucosidases.

Background References

1. Yang C et al. Celastrol increases glucocerebrosidase activity in Gaucher disease by modulating molecular chaperones. Proc Natl Acad Sci U S A 111:249-54 (2014).

2. McNeill A et al. Ambroxol improves lysosomal biochemistry in glucocerebrosidase mutation-linked Parkinson disease cells. Brain 137:1481-95 (2014).

Sequence Similarity

Belongs to the glycosyl hydrolase 30 family.

Subcellular Location

Lysosome membrane.

UNIPROT

SwissProt: P04062 Human

SwissProt: P17439 Mouse

Entrez Gene:684536 Rat

Synonyms

Acid beta glucosidase antibody

Acid beta-glucosidase antibody

Alglucerase antibody

Beta glucocerebrosidase antibody

BETA GLUCOSIDASE, ACID antibody

Beta-glucocerebrosidase antibody

betaGC antibody

D glucosyl N acylsphingosine glucohydrolase antibody

D-glucosyl-N-acylsphingosine glucohydrolase antibody

EC 3.2.1.45 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />Western blot analysis of GBA with anti-GBA antibody [JM10-76] (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1:1,000 dilution.<br />Lane 1: Wild-type HEK293T whole cell lysate (20 µg).<br />Lane 2: GBA knockout HEK293T whole cell lysate (20 µg).<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    Western blot analysis of GBA with anti-GBA antibody [JM10-76] (ET1703-32) at 1:1,000 dilution.
    Lane 1: Wild-type HEK293T whole cell lysate (20 µg).
    Lane 2: GBA knockout HEK293T whole cell lysate (20 µg).

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-32, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of GBA on U-87 MG cell lysates with Rabbit anti-GBA antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/2,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GBA on U-87 MG cell lysates with Rabbit anti-GBA antibody (ET1703-32) at 1/2,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-32) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GBA antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GBA antibody (ET1703-32) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-32) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GBA antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GBA antibody (ET1703-32) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-32) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GBA antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GBA antibody (ET1703-32) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-32) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-32" style="font-weight: bold;text-decoration: underline;">ET1703-32</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"