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AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98]

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Specification

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Catalog# ET1703-34

AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human AQP1 aa 245-269 / 269.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P, mIHC

Molecular Weight

Predicted band size: 28 kDa

Positive Control

Human lung tissue lysate, rat kidney tissue lysate, rat lung tissue lysate, mouse kidney tissue lysate, mouse lung tissue lysate, MCF-7, Hela, SW480, human spleen tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JM10-98

RRID

AB_3070391

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IF-Tissue

  • 1:500-1:2,000

  • IHC-P

  • 1:5,000

  • mIHC

  • 1:5,000-1:6,000

Target

Function

Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of Aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most Aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP1 is an integral membrane protein expressed in erythrocytes and renal tubule cells.

Background References

1. Lobo NC et al. Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine. BMC Cancer 16:485 (2016).

2. Méndez-Gómez HR et al. Transcytosis in the blood-cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system. Mol Ther Methods Clin Dev 2:15037 (2015).

Sequence Similarity

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Tissue Specificity

Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P29972 Human

SwissProt: Q02013 Mouse

SwissProt: P29975 Rat

Synonyms

AQP 1 antibody

AQP CHIP antibody

AQP-1 antibody

AQP1 antibody

AQP1_HUMAN antibody

aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody

aquaporin 1 (Colton blood group) antibody

Aquaporin CHIP antibody

Aquaporin-1 antibody

Aquaporin-CHIP antibody

Expand

Images

  • Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human lung tissue lysate<br />Lane 2: Rat kidney tissue lysate<br />Lane 3: Rat kidney tissue lysate (no heat)<br />Lane 4: Rat lung tissue lysate<br />Lane 5: Rat lung tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28~40 kDa<br />Exposure time: 6 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (ET1703-34) at 1/2,000 dilution.

    Lane 1: Human lung tissue lysate
    Lane 2: Rat kidney tissue lysate
    Lane 3: Rat kidney tissue lysate (no heat)
    Lane 4: Rat lung tissue lysate
    Lane 5: Rat lung tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 28~40 kDa
    Exposure time: 6 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse kidney tissue lysate<br />Lane 2: Mouse kidney tissue lysate (no heat)<br />Lane 3: Mouse lung tissue lysate<br />Lane 4: Mouse lung tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28~40 kDa<br />Exposure time: 24 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (ET1703-34) at 1/5,000 dilution.

    Lane 1: Mouse kidney tissue lysate
    Lane 2: Mouse kidney tissue lysate (no heat)
    Lane 3: Mouse lung tissue lysate
    Lane 4: Mouse lung tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 28~40 kDa
    Exposure time: 24 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-34) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NPHS2 (<a href="/products/ET7107-34" style="font-weight: bold;text-decoration: underline;">ET7107-34</a>, Red), anti-AQP1 (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>, Green), anti-Laminin beta 1 (<a href="/products/ET1703-14" style="font-weight: bold;text-decoration: underline;">ET1703-14</a>, Cyan) and anti-aSMA (<a href="/products/ET1607-53" style="font-weight: bold;text-decoration: underline;">ET1607-53</a>, Magenta) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit&trade;MH010101, www.luminiris.cn). The section was incubated in four rounds of staining: in the order of <a href="/products/ET7107-34" style="font-weight: bold;text-decoration: underline;">ET7107-34</a> (1/1,000 dilution), <a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a> (1/5,000 dilution), <a href="/products/ET1703-14" style="font-weight: bold;text-decoration: underline;">ET1703-14</a> (1/1,000 dilution) and <a href="/products/ET1607-53" style="font-weight: bold;text-decoration: underline;">ET1607-53</a> (1/10,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NPHS2 (ET7107-34, Red), anti-AQP1 (ET1703-34, Green), anti-Laminin beta 1 (ET1703-14, Cyan) and anti-aSMA (ET1607-53, Magenta) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in four rounds of staining: in the order of ET7107-34 (1/1,000 dilution), ET1703-34 (1/5,000 dilution), ET1703-14 (1/1,000 dilution) and ET1607-53 (1/10,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-AQP1 (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>, Violet), anti-CK18 (<a href="/products/ET1603-8" style="font-weight: bold;text-decoration: underline;">ET1603-8</a>, Green) and anti-Calbindin (<a href="/products/ET1702-54" style="font-weight: bold;text-decoration: underline;">ET1702-54</a>, Yellow) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit&trade;MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of <a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a> (1/5,000 dilution), <a href="/products/ET1603-8" style="font-weight: bold;text-decoration: underline;">ET1603-8</a> (1/3,000 dilution) and <a href="/products/ET1702-54" style="font-weight: bold;text-decoration: underline;">ET1702-54</a> (1/4,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

    Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-AQP1 (ET1703-34, Violet), anti-CK18 (ET1603-8, Green) and anti-Calbindin (ET1702-54, Yellow) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1703-34 (1/5,000 dilution), ET1603-8 (1/3,000 dilution) and ET1702-54 (1/4,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AQP1 antibody (ET1703-34) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-34) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AQP1 antibody (ET1703-34) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-34) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-AQP1 antibody (ET1703-34) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-34) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-AQP1 antibody (ET1703-34) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-34) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-AQP1 antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-34" style="font-weight: bold;text-decoration: underline;">ET1703-34</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-AQP1 antibody (ET1703-34) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-34) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: kidney<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Mouse

    Site: kidney

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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