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SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]

Usd: 385

Specification

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Catalog# ET1703-35

SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SMC3 aa 1994-1217 / 1217.

Species Reactivity

Human, Mouse, Rat, Zebrafish

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Target Molecular Weight

Predicted band size: 142 kDa

Positive Control

HepG2 cell lysate, NIH-3T3 cell lysate, PC12 cell lysate, Hela, HepG2, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse colon tissue, zebrafish tissue.

Conjugation

unconjugated

Clone Number

JM10-75

RRID

AB_3070392

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.

Background References

1. Kracker S et al. An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. J Allergy Clin Immunol 135:998-1007.e6 (2015).

2. Qiu Z et al. Functional interactions between NURF and Ctcf regulate gene expression. Mol Cell Biol 35:224-37 (2015).

Sequence Similarity

Belongs to the SMC family. SMC3 subfamily.

Post-translational Modification

Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation.; Phosphorylated at Ser-1083 in a SPO11-dependent manner.; Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.

Subcellular Location

Centromere, Chromosome, Nucleus.

UNIPROT

SwissProt: Q9UQE7 Human

SwissProt: Q9CW03 Mouse

SwissProt: P97690 Rat

Synonyms

BAM antibody

Bamacan antibody

Basement membrane associated chondroitin proteoglycan antibody

Basement membrane-associated chondroitin proteoglycan antibody

BMH antibody

CDLS3 antibody

chondroitin sulfate proteoglycan 6 (bamacan) antibody

Chondroitin sulfate proteoglycan 6 antibody

Chromosome associated polypeptide antibody

Chromosome-associated polypeptide antibody

Expand

Images

  • Western blot analysis of SMC3 on HepG2 lysates with Rabbit anti-SMC3 antibody (<a href="/products/ET1703-35" style="font-weight: bold;text-decoration: underline;">ET1703-35</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1703-35" style="font-weight: bold;text-decoration: underline;">ET1703-35</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 141.5kDa<br />Observed band size: 142 kDa

    Western blot analysis of SMC3 on HepG2 lysates with Rabbit anti-SMC3 antibody (ET1703-35) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1703-35, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 141.5kDa
    Observed band size: 142 kDa

  • ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"