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Perilipin A Recombinant Rabbit Monoclonal Antibody [JM10-96]

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Specification

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Catalog# ET1703-38

Perilipin A Recombinant Rabbit Monoclonal Antibody [JM10-96]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Predicted reactivity

  • Mouse

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Perilipin A Recombinant Rabbit Monoclonal Antibody [JM10-96]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Perilipin A aa 470-510 / 522.

Species Reactivity

Human (Predicted: Mouse)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 56 kDa

Positive Control

Human liver tissue lysates, MCF-7, HepG2, Hela.

Conjugation

unconjugated

Clone Number

JM10-96

RRID

AB_3070395

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100-1:500

Target

Function

Perilipin is a protein that coats lipid droplets (LDs) in adipocytes, the fat-storing cells in adipose tissue. In fact, PLIN1 is greatly expressed in white adipocytes.It controls adipocyte lipid metabolism. It handles essential functions in the regulation of basal and hormonally stimulated lipolysis and also rises the formation of large LDs which implies an increase in the synthesis of triglycerides.In humans, Perilipin A is the most abundant protein associated with the adipocyte LDs and lower PLIN1 expression is related with higher rates of lipolysis.Under basal conditions, Perilipin acts as a protective coating of LDs from the body's natural lipases, such as hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), which break triglycerides into glycerol and free fatty acids for use in lipid metabolism.In times of energy deficit, Perilipin is hyperphosphorylated by PKA following β-adrenergic receptor activation. Phosphorylated perilipin changes conformation, exposing the stored lipids to hormone-sensitive lipase-mediated lipolysis.

Background References

1. Matthews AG et al. Compound heterozygous mutation of Rag1 leading to Omenn syndrome. PLoS One 10:e0121489 (2015).

2. Nurnberg ST et al. Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap. PLoS Genet 11:e1005155 (2015).

Sequence Similarity

Belongs to the perilipin family.

Tissue Specificity

Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.

Post-translational Modification

Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity).

Subcellular Location

Endoplasmic reticulum, Lipid droplet.

UNIPROT

SwissProt: O60240 Human

SwissProt: Q8CGN5 Mouse

Synonyms

Lipid droplet associated protein antibody

Lipid droplet-associated protein antibody

PERI antibody

Perilipin A antibody

Perilipin antibody

Perilipin-1 antibody

PerilipinA antibody

PLIN antibody

PLIN1 antibody

PLIN1_HUMAN antibody

Expand

Images

  • Western blot analysis of Perilipin A on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-38" style="font-weight: bold;text-decoration: underline;">ET1703-38</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Perilipin A on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Perilipin A in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1703-38" style="font-weight: bold;text-decoration: underline;">ET1703-38</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Perilipin A in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Perilipin A in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1703-38" style="font-weight: bold;text-decoration: underline;">ET1703-38</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Perilipin A in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Perilipin A in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1703-38" style="font-weight: bold;text-decoration: underline;">ET1703-38</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Perilipin A in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation