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CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40]

Usd: 385

Specification

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Catalog# ET1703-41

CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD73 Recombinant Rabbit Monoclonal Antibody [JM11-40]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD73 aa 491-536 / 574.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 63 kDa

Positive Control

HT-29 cell lysate, U-87 MG cell lysate, HepG2 cell lysate, A375 cell lysate, human endometrium tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

JM11-40

RRID

AB_3070398

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:50-1:200

Target

Function

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

Background References

1. Ruiz EJ et al. A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Nat Commun 5:3175 (2014).

2. H usler SF et al. Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion. Am J Transl Res 6:129-39 (2014).

Sequence Similarity

Belongs to the 5'-nucleotidase family.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P21589 Human

Synonyms

5' NT antibody

5' nucleotidase (CD73) antibody

5' nucleotidase precursor antibody

5' nucleotidase, ecto antibody

5' nucleotidase, ecto (CD73) antibody

5'-NT antibody

5'-nucleotidase antibody

5NTD_HUMAN antibody

CD73 antibody

CD73 antigen antibody

Expand

Images

  • Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/2,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: U-87 MG cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (ET1703-41) at 1/2,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: U-87 MG cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 70 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/2,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: A375 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 5 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (ET1703-41) at 1/2,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: A375 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 70 kDa

    Exposure time: 5 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD73 antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD73 antibody (ET1703-41) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD73 antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-41" style="font-weight: bold;text-decoration: underline;">ET1703-41</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD73 antibody (ET1703-41) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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