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Cathepsin L/V/K/H Recombinant Rabbit Monoclonal Antibody [JM10-78]

Usd: 385

Specification

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Catalog# ET1703-44

Cathepsin L/V/K/H Recombinant Rabbit Monoclonal Antibody [JM10-78]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cathepsin L/V/K/H Recombinant Rabbit Monoclonal Antibody [JM10-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cathepsin aa 92-138 / 333.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 37/38 kDa

Positive Control

HepG2 cell lysate, A549 cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JM10-78

RRID

AB_3070401

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Tissue

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

Target

Function

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin L (also designated major excreted protein, MEP or CATL) is a member of the peptidase C1 family and has been identified as a protein that is most closely related to cathepsin H. It is a lysosomal cysteine proteinase that mediates intracellular protein catabolism for collagen, elastin and ?-1 protease inhibitor. Cathepsin L is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Transformed mouse fibroblasts stimulated by growth factors or tumor promoters secrete a form of cathepsin L.

Background References

1. Huang J et al. Effect of a low-protein diet supplemented with ketoacids on skeletal muscle atrophy and autophagy in rats with type 2 diabetic nephropathy. PLoS One 8:e81464 (2013).

Sequence Similarity

Belongs to the peptidase C1 family.

Subcellular Location

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Nucleus, Secreted.

UNIPROT

SwissProt: P07711 Human

SwissProt: O60911 Human

SwissProt: P43235 Human

SwissProt: P09668 Human

Synonyms

Cathepsin L antibody

cathepsin L

1 b antibody

Cathepsin L1 antibody

Cathepsin L1 light chain antibody

CathepsinL antibody

CATL antibody

CATL1_HUMAN antibody

cb15 antibody

CTSL antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cathepsin L/V/K/H on different lysates with Rabbit anti-Cathepsin L/V/K/H antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Cathepsin H cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 37/38 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cathepsin L/V/K/H on different lysates with Rabbit anti-Cathepsin L/V/K/H antibody (ET1703-44) at 1/1,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Cathepsin H cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 37/38 kDa
    Observed band size: 42 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cathepsin L/V/K/H on HCT 116 cell lysate with Rabbit anti-Cathepsin L/V/K/H antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/2,000 dilution.<br /><br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 37/38 kDa<br />Observed band size: 56 kDa

    Western blot analysis of Cathepsin L/V/K/H on HCT 116 cell lysate with Rabbit anti-Cathepsin L/V/K/H antibody (ET1703-44) at 1/2,000 dilution.


    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1703-44, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 37/38 kDa
    Observed band size: 56 kDa

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L/V/K/H antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cathepsin L/V/K/H antibody (ET1703-44) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-44) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cathepsin L/V/K/H antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cathepsin L/V/K/H antibody (ET1703-44) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-44) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Cathepsin L/V/K/H antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-44" style="font-weight: bold;text-decoration: underline;">ET1703-44</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Cathepsin L/V/K/H antibody (ET1703-44) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-44) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation