FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09]
Catalog# ET1703-55
FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human FKBP12 aa 76-108 / 108.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 12 kDa
Positive Control
SH-SY5Y cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JM11-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases which bind to and mediate the effects of immunosuppressive drugs such as Cyclosporin, FK506 and Rapamycin. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid immunophilin belonging to the FKBP-type PPIase family. Subcellularly localized to the cytoplasm, FKBP12.6 binds to RyR in cardiac muscle sarcoplasmic reticulum and possibly plays a unique physiological role in excitation-contraction coupling in cardiac muscle. FKBP12.6 also catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Ubiquitously expressed, FKBP12.6 is found at highest levels in brain and thymus. FKBP12.6 is expressed as two isoforms produced by alternative splicing.
Background References
1. Ballister ER et al. Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint. J Cell Biol 204:901-8 (2014).
2. Buheitel J & Stemmann O Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate. EMBO J 32:666-76 (2013).
Sequence Similarity
Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
Subcellular Location
Cytoplasm, Membrane, Sarcoplasmic reticulum.
Synonyms
12 kDa FK506-binding protein antibody
12 kDa FKBP antibody
Calstabin 1 antibody
FK506 binding protein 1 antibody
FK506 binding protein 12 antibody
FK506 binding protein 1A (12kD) antibody
FK506 binding protein 1A 12kDa antibody
FK506 binding protein 1A antibody
FK506 binding protein T cell 12 kD antibody
FK506 binding protein, T cell, 12 kD antibody
ExpandImages
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Western blot analysis of FKBP12 on different lysates with Rabbit anti-FKBP12 antibody (ET1703-55) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 14 kDa
Exposure time: 46 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"