PABPN1 Recombinant Rabbit Monoclonal Antibody [JM11-28]
Catalog# ET1703-66
PABPN1 Recombinant Rabbit Monoclonal Antibody [JM11-28]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PABPN1 Recombinant Rabbit Monoclonal Antibody [JM11-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PABPN1 aa 4-51 / 306.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
Hep G2 (Human hepatocellular carcinoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, AGS, SW480, mouse testis tissue, Hela.
Conjugation
unconjugated
Clone Number
JM11-28
RRID
Reactivity Data
| WB | IF-Cell | IF-Tissue | IHC-P | FC | IP | |
|---|---|---|---|---|---|---|
| Human |
|
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| Mouse | ||||||
| Rat |
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
Target
Function
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
Background References
1. Bergeron D et al. Regulated Intron Retention and Nuclear Pre-mRNA Decay Contribute to PABPN1 Autoregulation. Mol Cell Biol 35:2503-17 (2015).
2. Davidson L et al. 3\' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells. Genes Dev 28:342-56 (2014).
Tissue Specificity
Ubiquitous.
Post-translational Modification
Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
Nuclear poly(A)-binding protein 1 antibody
OPMD antibody
PAB2 antibody
PABII antibody
PABP 2 antibody
pABP-2 antibody
PABP2 antibody
PABP2_HUMAN antibody
PABPII antibody
Pabpn1 antibody
ExpandImages
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Western blot analysis of PABPN1 on different lysates with Rabbit anti-PABPN1 antibody (ET1703-66) at 1/5,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1703-66, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 33 kDa
Observed band size: 55 kDa -
ICC staining of PABPN1 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-66, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of PABPN1 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-66, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PABPN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of PABPN1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"