Endothelin B Receptor Recombinant Rabbit Monoclonal Antibody [JM74-10]
Catalog# ET1703-79
Endothelin B Receptor Recombinant Rabbit Monoclonal Antibody [JM74-10]
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WB
-
IP
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Human
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Zebrafish
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unconjugated
Overview
Product Name
Endothelin B Receptor Recombinant Rabbit Monoclonal Antibody [JM74-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Endothelin B Receptor aa 58-91 / 442.
Species Reactivity
Human, Zebrafish
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 50 kDa
Positive Control
Zebrafish tissue lysate, Raji cell lysate, JAR cell lysate.
Conjugation
unconjugated
Clone Number
JM74-10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
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IP
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1:10-1:50
Target
Function
Endothelin receptor B (ETBR), also known as EDNRB, ETB, ETRB, HSCR and HSCR2, is a member of the guanine-binding, regulatory protein-coupled receptor family. Three isoforms of ETBR exist called isoform 1, isoform 2 and δ 3. ETBR is involved in the regulation of sodium excretion and glomular filtration rate (GFR). ETBR plays a role in the normal development of the neural crestderived cell lineages, epidermal melanocytes and enteric neurons. ETBR is expressed in lung, kidney, placenta, skeletal muscle and stem villi vessels. Both of the ET receptors, ETAR and ETBR, are activated by ET1, which results in inhibition of active lens sodium-potassium transport. Activation of the ET receptors also causes an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. ETBR deficiency causes early onset dysfunction of the kidney, characterized by reduced sodium excretion, decreased GFR and slightly elevated blood pressure. Mutations in the gene encoding ETBR produce congenital aganglionic megacolon and pigment abnormalities. The multigenic disorder, Hirschsprung’s disease type 2, is also due to a mutation in the ETBR gene.
Background References
1. Tagashira H et al. UVB Stimulates the Expression of Endothelin B Receptor in Human Melanocytes via a Sequential Activation of the p38/MSK1/CREB/MITF Pathway Which Can Be Interrupted by a French Maritime Pine Bark Extract through a Direct Inactivation of MSK1. PLoS One 10:e0128678 (2015).
2. Hall SM et al. Endothelin receptor expression in idiopathic pulmonary arterial hypertension: effect of bosentan and epoprostenol treatment. Eur Respir J 38:851-60 (2011).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
Tissue Specificity
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
Post-translational Modification
Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
ABCDS antibody
Ednra antibody
EDNRB antibody
EDNRB_HUMAN antibody
Endothelin B receptor antibody
Endothelin B receptor precursor antibody
Endothelin receptor Non selective type antibody
Endothelin receptor non-selective type antibody
Endothelin receptor type B antibody
ET B antibody
ExpandImages
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Western blot analysis of Endothelin B Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Zebrafish tissue lysate
Lane 2: Raji cell lysate
Lane 3: JAR cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"