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Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]

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Specification

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Catalog# ET1703-96

Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Axin2 aa 55-93 / 843.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC(Intra)

Target Molecular Weight

Predicted band size: 94 kDa

Positive Control

MDA-MB-231 (Human breast cancer cell) cell lysate, SW620 (Human colon cancer cell) cell lysate, SW480 (Human colorectal zdenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate.

Conjugation

unconjugated

Clone Number

JM11-30

RRID

AB_3070450

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC(Intra) IF-Cell
human
Tested Tested
Tested Tested
Not Recommended
Not Recommended
mouse
Tested Tested
Tested Tested
Tested Tested
Tested Tested
rat
Tested Tested
 
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:1,000

  • FC(Intra)

  • 1:2,000

Target

Function

b-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of b-catenin, by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor-T cell factor family and modulation of gene expression. Axin is involved in the degradation of b-catenin by acting as a scaffold to form a complex between b-catenin, adenomatous polyposis coli (APC) and GSK-3b. APC, which is phosphorylated by GSK-3b, induces degradation of b-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to axin and appears to play a similar role to axin in the Wnt signaling pathway.

Background References

1. Ng OH et al. Deregulated WNT signaling in childhood T-cell acute lymphoblastic leukemia. Blood Cancer J 4:e192 (2014).

2. Winkler T et al. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9:e98444 (2014).

Tissue Specificity

Expressed in brain and lymphoblast.

Post-translational Modification

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.; ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.; Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9Y2T1 Human

SwissProt: O88566 Mouse

SwissProt: O70240 Rat

Synonyms

Axil antibody

Axin like protein antibody

Axin-2 antibody

Axin-like protein antibody

Axin2 antibody

AXIN2_HUMAN antibody

Axis inhibition protein 2 antibody

Conductin antibody

DKFZp781B0869 antibody

MGC10366 antibody

Expand

Images

  • Western blot analysis of Axin2 on different lysates with Rabbit anti-Axin2 antibody (<a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>) at 1/5,000 dilution.<br /><br />Lane 1: MDA-MB-231 (Human breast cancer cell) cell lysate<br />Lane 2: SW620 (Human colon cancer cell) cell lysate<br />Lane 3: SW480 (Human colorectal zdenocarcinoma cell) cell lysate<br />Lane 4: MCF7 (Human breast cancer cell) cell lysate<br />Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 6: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 8: C6 (Rat glioma cell) cell lysate<br />Lane 9: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 94 kDa<br />Observed band size: 94 kDa

    Western blot analysis of Axin2 on different lysates with Rabbit anti-Axin2 antibody (ET1703-96) at 1/5,000 dilution.

    Lane 1: MDA-MB-231 (Human breast cancer cell) cell lysate
    Lane 2: SW620 (Human colon cancer cell) cell lysate
    Lane 3: SW480 (Human colorectal zdenocarcinoma cell) cell lysate
    Lane 4: MCF7 (Human breast cancer cell) cell lysate
    Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 6: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 8: C6 (Rat glioma cell) cell lysate
    Lane 9: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1703-96, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 94 kDa
    Observed band size: 94 kDa

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Mouse <br />Sample: NIH/3T3 (Mouse fibroblast)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br /><br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/100, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature.<br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblast)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: ET1703-96, 1/100, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Lung cancer<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Lung cancer
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Colon<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Lung<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Lung
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Colon<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Lung<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Lung
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Colon<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Lung<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Lung
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1703-96, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Mouse<br />Sample: NIH/3T3 (Mouse fibroblasts)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/ET1703-96" style="font-weight: bold;text-decoration: underline;">ET1703-96</a>(1/2,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Mouse
    Sample: NIH/3T3 (Mouse fibroblasts)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: ET1703-96(1/2,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"