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MINA53 Recombinant Rabbit Monoclonal Antibody [JA11-63]

Usd: 385

Specification

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Catalog# ET1704-07

MINA53 Recombinant Rabbit Monoclonal Antibody [JA11-63]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

MINA53 Recombinant Rabbit Monoclonal Antibody [JA11-63]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MINA53 aa 416-465 / 465.

Species Reactivity

Human, Mouse

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 53 kDa

Positive Control

HDLM-2 cell lysate, HUT 102 cell lysate, KG-1 cell lysate, A431 cell lysate, A549 cell lysate, Mouse testis tissue lysate, Mouse placenta (E16.5) tissue lysate.

Conjugation

unconjugated

Clone Number

JA11-63

RRID

AB_3070460

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.

Background References

1. Ishizaki, H., Yano, H., Tsuneoka, M., Ogasawara, S., Akiba, J., Nishida, N., Kojiro, S., Fukahori, S., Moriya, F., Matsuoka, K. and Kojiro, M. 2007. Overexpression of the Myc target gene Mina53 in advanced renal cell carcinoma. Pathol. Int. 57: 672-680.

2. Eilbracht, J., Kneissel, S., Hofmann, A. and Schmidt-Zachmann, M.S. 2005. Protein NO52—a constitutive nucleolar component sharing high sequence homologies to protein NO66. Eur. J. Cell Biol. 84: 279-294.

Sequence Similarity

Belongs to the ROX family. MINA53 subfamily.

Tissue Specificity

Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q8IUF8 Human

SwissProt: Q8CD15 Mouse

Synonyms

60S ribosomal protein L27a histidine hydroxylase antibody

Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody

FLJ14393 antibody

Histone lysine demethylase MINA antibody

MDIG antibody

MINA antibody

MINA_HUMAN antibody

Mineral dust-induced gene protein antibody

Myc induced nuclear antigen 53 kDa antibody

MYC induced nuclear antigen antibody

Expand

Images

  • Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (<a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a>) at 1/2,000 dilution.<br /><br />Lane 1: HDLM-2 cell lysate<br />Lane 2: HUT 102 cell lysate<br />Lane 3: KG-1 cell lysate<br />Lane 4: A431 cell lysate<br />Lane 5: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (ET1704-07) at 1/2,000 dilution.

    Lane 1: HDLM-2 cell lysate
    Lane 2: HUT 102 cell lysate
    Lane 3: KG-1 cell lysate
    Lane 4: A431 cell lysate
    Lane 5: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (<a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse testis tissue lysate<br />Lane 2: Mouse placenta (E16.5) tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 13 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MINA53 on different lysates with Rabbit anti-MINA53 antibody (ET1704-07) at 1/2,000 dilution.

    Lane 1: Mouse testis tissue lysate
    Lane 2: Mouse placenta (E16.5) tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 13 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-07) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • MINA53 was immunoprecipitated from 0.2 mg A549 cell lysate with <a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A549 cell lysate (input)<br />Lane 2: <a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a> IP in A549 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1704-07" style="font-weight: bold;text-decoration: underline;">ET1704-07</a> in A549 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 13 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    MINA53 was immunoprecipitated from 0.2 mg A549 cell lysate with ET1704-07 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1704-07 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A549 cell lysate (input)
    Lane 2: ET1704-07 IP in A549 cell lysate
    Lane 3: Rabbit IgG instead of ET1704-07 in A549 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 13 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"