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Vitamin D Receptor Recombinant Rabbit Monoclonal Antibody [JA11-16]

Usd: 385

Specification

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Catalog# ET1704-09

Vitamin D Receptor Recombinant Rabbit Monoclonal Antibody [JA11-16]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Vitamin D Receptor Recombinant Rabbit Monoclonal Antibody [JA11-16]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide with Human Vitamin D Receptor aa 1-50 / 427.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 48 kDa

Positive Control

HL-60 cell lysate, T-47D cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, Hela WT cell lysate, rat large intestine tissue, human skin tissue, mouse colon tissue, mouse skin tissue, Hela, rat skin tissue.

Conjugation

unconjugated

Clone Number

JA11-16

RRID

AB_2923198

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

This gene encodes the nuclear hormone receptor for vitamin D3. The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins.

Background References

1. Lungchukiet P et al. Suppression of epithelial ovarian cancer invasion into the omentum by 1a,25-dihydroxyvitamin D3 and its receptor. J Steroid Biochem Mol Biol 148:138-47 (2015).

2. Kasiappan R et al. Vitamin D suppresses leptin stimulation of cancer growth through microRNA. Cancer Res 74:6194-204 (2014).

Sequence Similarity

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P11473 Human

SwissProt: P48281 Mouse

SwissProt: P13053 Rat

Synonyms

1 25 dihydroxyvitamin D3 receptor antibody

1 antibody

1,25 dihydroxyvitamin D3 receptor antibody

1,25-@dihydroxyvitamin D3 receptor antibody

25-dihydroxyvitamin D3 receptor antibody

Member 1 antibody

NR1I1 antibody

Nuclear receptor subfamily 1 group I member 1 antibody

PPP1R163 antibody

Protein phosphatase 1, regulatory subunit 163 antibody

Expand

Images

  • Western blot analysis of Vitamin D Receptor on different lysates with Rabbit anti-Vitamin D Receptor antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1/5,000 dilution.<br /><br />Lane 1: HL-60 cell lysate (15 µg/Lane)<br />Lane 2: T-47D cell lysate (15 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (15 µg/Lane)<br />Lane 4: L-929 cell lysate (15 µg/Lane)<br />Lane 5: Rat kidney tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Vitamin D Receptor on different lysates with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/5,000 dilution.

    Lane 1: HL-60 cell lysate (15 µg/Lane)
    Lane 2: T-47D cell lysate (15 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
    Lane 4: L-929 cell lysate (15 µg/Lane)
    Lane 5: Rat kidney tissue lysate (20 µg/Lane)

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-09) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of Vitamin D Receptor with anti-Vitamin D Receptor antibody[JA11-16] (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1:500 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2/3: Vitamin D Receptor knockout Hela whole cell lysate (10 µg).<br /><br /><a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a> was shown to specifically react with Vitamin D Receptor in wild-type Hela cells. No bands were observed when Vitamin D Receptor knockout sample were tested. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of Vitamin D Receptor with anti-Vitamin D Receptor antibody[JA11-16] (ET1704-09) at 1:500 dilution.
    Lane 1: Wild-type Hela whole cell lysate (10 µg).
    Lane 2/3: Vitamin D Receptor knockout Hela whole cell lysate (10 µg).

    ET1704-09 was shown to specifically react with Vitamin D Receptor in wild-type Hela cells. No bands were observed when Vitamin D Receptor knockout sample were tested. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Vitamin D Receptor antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-09, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Vitamin D Receptor antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1704-09" style="font-weight: bold;text-decoration: underline;">ET1704-09</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Vitamin D Receptor antibody (ET1704-09) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-09) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation