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Rhodopsin Recombinant Rabbit Monoclonal Antibody [JA10-91]

Usd: 385

Specification

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Catalog# ET1704-12

Rhodopsin Recombinant Rabbit Monoclonal Antibody [JA10-91]

Application

  • WB

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Rhodopsin Recombinant Rabbit Monoclonal Antibody [JA10-91]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Rhodopsin aa 299-348 / 348.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr

Target Molecular Weight

39 kDa

Positive Control

Rat retina tissue.

Conjugation

unconjugated

Clone Number

JA10-91

RRID

AB_3070464

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:200-1:500

Target

Function

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

Background References

1. Sawant O et al. Light-Regulated Thyroid Hormone Signaling Is Required for Rod Photoreceptor Development in the Mouse Retina. Invest Ophthalmol Vis Sci 56:8248-57 (2015).

2. Wavre-Shapton ST et al. Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium. J Cell Sci 127:3852-61 (2014).

Sequence Similarity

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Tissue Specificity

Rod shaped photoreceptor cells which mediate vision in dim light.

Post-translational Modification

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region (By similarity). After activation by light, phosphorylated by GRK1 (in vitro).; Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal(PubMed:12566452).

Subcellular Location

Membrane.

UNIPROT

SwissProt: P08100 Human

SwissProt: P51489 Rat

Synonyms

CSNBAD1 antibody

MGC138309 antibody

MGC138311 antibody

OPN 2 antibody

OPN2 antibody

opsd antibody

OPSD_HUMAN antibody

opsin 2 antibody

Opsin 2 rod pigment antibody

Opsin-2 antibody

Expand

Images

  • Western blot analysis of Rhodopsin on different lysates with Rabbit anti-Rhodopsin antibody (<a href="/products/ET1704-12" style="font-weight: bold;text-decoration: underline;">ET1704-12</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat eye tissue lysate<br />Lane 2: Rat liver tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 1 minute; ECL: K1801<br /><br />The multiple bands correspond to dimers and multimers of Rhodopsin, consistent with the literature (PMID: 25270370). Negative expression of Rhodopsin protein in liver tissue is consistent with the predicted expression pattern.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1704-12" style="font-weight: bold;text-decoration: underline;">ET1704-12</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Western blot analysis of Rhodopsin on different lysates with Rabbit anti-Rhodopsin antibody (ET1704-12) at 1/1,000 dilution.

    Lane 1: Rat eye tissue lysate
    Lane 2: Rat liver tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 1 minute; ECL: K1801

    The multiple bands correspond to dimers and multimers of Rhodopsin, consistent with the literature (PMID: 25270370). Negative expression of Rhodopsin protein in liver tissue is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1704-12, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Retina<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1704-12" style="font-weight: bold;text-decoration: underline;">ET1704-12</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Retina
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1704-12, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Retina<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/ET1704-12" style="font-weight: bold;text-decoration: underline;">ET1704-12</a>, 1/1,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Retina
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: ET1704-12, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Retina<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br /><br />Primary antibody: <a href="/products/ET1704-12" style="font-weight: bold;text-decoration: underline;">ET1704-12</a>, 1/200, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Retina
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: ET1704-12, 1/200, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Rat<br />Tissue: Retina<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br /><br />Primary antibody: <a href="/products/ET1704-12" style="font-weight: bold;text-decoration: underline;">ET1704-12</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Retina
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: ET1704-12, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"