SFRP1 Recombinant Rabbit Monoclonal Antibody [JA11-68]
Catalog# ET1704-29
SFRP1 Recombinant Rabbit Monoclonal Antibody [JA11-68]
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WB
-
IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
SFRP1 Recombinant Rabbit Monoclonal Antibody [JA11-68]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SFRP1 aa 31-80 / 314.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 35 kDa
Positive Control
SH-SY5Y cell lysate, SK-MEL-28 cell lysate, NIH/3T3 cell lysate, Mouse kidney tissue lysate, human testis tissue.
Conjugation
unconjugated
Clone Number
JA11-68
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
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1:1,000
Target
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Background References
1. Zuckermann M et al. Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nat Commun 6:7391 (2015).
2. Cyr AR et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014).
Sequence Similarity
Belongs to the secreted frizzled-related protein (sFRP) family.
Tissue Specificity
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
Subcellular Location
Secreted.
Synonyms
Frizzled related protein 1 antibody
FRP 1 antibody
FRP antibody
FRP-1 antibody
FRP1 antibody
FrzA antibody
SARP 2 antibody
SARP-2 antibody
SARP2 antibody
Secreted apoptosis related protein 2 antibody
ExpandImages
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Western blot analysis of SFRP1 on different lysates with Rabbit anti-SFRP1 antibody (ET1704-29) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SFRP1 antibody (ET1704-29) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"