Glucose Transporter GLUT3 Recombinant Rabbit Monoclonal Antibody [JA50-31]
Overview
Product Name
Glucose Transporter GLUT3 Recombinant Rabbit Monoclonal Antibody [JA50-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human GLUT3 aa 11-60 / 496.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 54 kDa
Positive Control
HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y.
Conjugation
unconjugated
Clone Number
JA50-31
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
FC
-
1:50-1:100
Target
Function
Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. Glut1, Glut3 and Glut4 are high-affinity transporters, whereas Glut2 is a low-affinity transporter. In adipose and muscle tissue, Insulin stimulates a rapid and dramatic increase in glucose uptake, largely due to the redistribution of the Insulin-inducible glucose transporter, Glut4. In response to Insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to Insulin. Glut2 expression is seen in pancreatic b cells, hepatocytes and basolateral membranes of intestinal and epithelial cells, while the highest expression of Glut3 has been found in neuronal tissue.
Background References
1. Zhu Y et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog 12:e1005648 (2016).
2. Tu H et al. Low Red Blood Cell Vitamin C Concentrations Induce Red Blood Cell Fragility: A Link to Diabetes Via Glucose, Glucose Transporters, and Dehydroascorbic Acid. EBioMedicine 2:1735-50 (2015).
Sequence Similarity
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Tissue Specificity
Highly expressed in brain. Expressed in many tissues.
Subcellular Location
Cell membrane. Perikaryon. Cell projection.
Synonyms
brain antibody
FLJ90380 antibody
Glucose transporter type 3 antibody
Glucose transporter type 3 brain antibody
GLUT 3 antibody
GLUT-3 antibody
GLUT3 antibody
GTR3_HUMAN antibody
Slc2a3 antibody
Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
ExpandImages
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Western blot analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody (ET1704-59) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: RAW264.7 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-59) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody (ET1704-59) at 1/1,000 dilution.
Lane 1: HepG2-si NT cell lysate (10 µg/Lane)
Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minute; ECL: merk
4-20% SDS-PAGE gel.
ET1704-59 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of Glucose Transporter GLUT3 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
SEMA4D/VEGF surface enhances endothelialization by diminished-glycolysis-mediated M2-like macrophage polarization
Journal: Materials Today Bio
DOI: 10.1016/j.mtbio.2023.100832
IF: 8.2
Application: WB
Reactivity: Human
Publish date: 2023 Oct
-
Activation of AMPK pathway compromises Rab11 downregulation-mediated inhibition of Schwann cell proliferation in a Glut1 and Glut3-dependent manner
Journal: Neuroscience Letters
DOI:
IF: 2.27
Application: WB
Reactivity: Rat
Publish date: 2020 Feb
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