DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
Catalog# ET1704-75
DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
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WB
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IHC-P
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Human
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Rat
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Mouse
Predicted species support after-sales service
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unconjugated
Overview
Product Name
DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within DAZL aa 10-59 / 295.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 33 kDa
Positive Control
Human testis tissue, rat testis tissue.
Conjugation
unconjugated
Clone Number
JA10-36
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:400
Target
Function
DAZL (deleted in azoospermia-like), an RNA-binding protein that effects spermatogenesis, is an autosomal homolog of the Y chromosome DAZ (deleted in azoospermia). The gene encoding human DAZL maps to chromosome locus 3p24. DAZL, like other members of The DAZ family members are included in germ cells in both male and female gonads. A Thr 54-to-Ala mutation within the RNA-recognition domain of DAZL proteins contributes to spermatogenic failure. Infertility in DAZL knockout catisatalities are unable to complete the first meiotic prophase in the first wave of spermatogenesis.
Background References
1. Wang H et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016).
2. Fleck D et al. Distinct purinergic signaling pathways in prepubescent mouse spermatogonia. J Gen Physiol 148:253-71 (2016).
Sequence Similarity
Belongs to the RRM DAZ family.
Tissue Specificity
Testis specific.
Subcellular Location
Cytoplasm. Nucleus. Predominantly cytoplasmic.
Synonyms
DAZ homolog antibody
DAZ like autosomal antibody
DAZ-like autosomal antibody
DAZH antibody
DAZL 1 antibody
DAZL antibody
DAZL_HUMAN antibody
DAZL1 antibody
DAZLA antibody
Deleted in azoospermia like 1 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DAZL antibody (ET1704-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DAZL antibody (ET1704-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"