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SPHK1 Recombinant Rabbit Monoclonal Antibody [JA31-14]

Usd: 385

Specification

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Catalog# ET1704-76

SPHK1 Recombinant Rabbit Monoclonal Antibody [JA31-14]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SPHK1 Recombinant Rabbit Monoclonal Antibody [JA31-14]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SPHK1 aa 1-200 / 384.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 43 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, Mouse lung tissue lysate.

Conjugation

unconjugated

Clone Number

JA31-14

RRID

AB_3070520

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P). Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. One potent inhibitor of SphK1 activity is DMS (N,N-dimethylsphingosine). SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival. Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFα. Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases. SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype.

Background References

1. Testai FD et al. Changes in the metabolism of sphingolipids after subarachnoid hemorrhage. J Neurosci Res 93:796-805 (2015).

2. Cai K et al. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 1841:552-62 (2014).

Tissue Specificity

Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level).

Subcellular Location

Cytoplasm. Nucleus. Membrane.

UNIPROT

SwissProt: Q9NYA1 Human

SwissProt: Q8CI15 Mouse

Synonyms

SK 1 antibody

SK1 antibody

Sphingosine kinase 1 antibody

SPHK 1 antibody

SPHK antibody

Sphk1 antibody

SPHK1_HUMAN antibody

SPK antibody

SPK 1 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SPHK1 on different lysates with Rabbit anti-SPHK1 antibody (<a href="/products/ET1704-76" style="font-weight: bold;text-decoration: underline;">ET1704-76</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: MCF7 cell lysate (20 µg/Lane)<br />Lane 4: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 5: Mouse lung tissue lysate (30 µg/Lane)<br />Lane 6: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane)<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43-51 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1704-76" style="font-weight: bold;text-decoration: underline;">ET1704-76</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SPHK1 on different lysates with Rabbit anti-SPHK1 antibody (ET1704-76) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: MCF7 cell lysate (20 µg/Lane)
    Lane 4: RAW264.7 cell lysate (20 µg/Lane)
    Lane 5: Mouse lung tissue lysate (30 µg/Lane)
    Lane 6: Mouse skeletal muscle tissue lysate (negative) (30 µg/Lane)

    Predicted band size: 43 kDa
    Observed band size: 43-51 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-76) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation