MUC4 Recombinant Rabbit Monoclonal Antibody [JM19-36]
Catalog# ET1705-13
MUC4 Recombinant Rabbit Monoclonal Antibody [JM19-36]
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WB
-
IHC-P
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Human
-
unconjugated
Overview
Product Name
MUC4 Recombinant Rabbit Monoclonal Antibody [JM19-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human MUC4 aa 4,688-5,412 / 5,412.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 542 kDa
Positive Control
Capan-1 (Human pancreatic cancer cell) cell lysate, SW 1990 (Human pancreatic cancer cell) cell lysates.
Conjugation
unconjugated
Clone Number
JM19-36
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
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IHC-P
-
1:100-1:1,000
Target
Function
Mucin 4 (MUC 4) is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, MUC-4 is a high-molecular weight glycoprotein. The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC-4 has been found to play various roles in the progression of cancer, particularly due to its signaling and anti-adhesive properties which contribute to tumor development and metastasis. It is also found to play roles in other diseases such as endometriosis and inflammatory bowel disease. MUC-4 belongs to the human mucin family that is membrane-anchored and can range in molecular weight from 550 to 930 kDa for the actual protein, and up to 4,650 kDa with glycosylation.
Background References
1. Tang J et al. The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer. J Exp Clin Cancer Res 35:91 (2016).
2. Lee H et al. DA-6034-induced mucin secretion via Ca2+-dependent pathways through P2Y receptor stimulation. Invest Ophthalmol Vis Sci 55:6565-74 (2014).
Tissue Specificity
Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
Post-translational Modification
Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.; mucrnin-4 alpha chain is highly O-glycosylated.; mucin-4 beta chain is predominantly N-glycosylated.
Subcellular Location
Secreted, Cell membrane and Membrane.
UNIPROT
Synonyms
Ascites sialoglycoprotein 1 antibody
Ascites sialoglycoprotein 2 antibody
Ascites sialoglycoprotein antibody
ASGP antibody
ASGP-1 antibody
ASGP-2 antibody
HSA276359 antibody
MUC 4 antibody
MUC-4 antibody
Muc4 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of MUC4 on different lysates with Rabbit anti-MUC4 antibody (ET1705-13) at 1/5,000 dilution.
Lane 1: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate
Lane 2: Capan-1 (Human pancreatic cancer cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 42 seconds; ECL: K1801
PANC-1 is a negative control (PMID : 23152882).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1705-13, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 542 kDa
Observed band size: 100-170 kDa -
Western blot analysis of MUC4 on SW 1990 (Human pancreatic cancer cell) cell lysate with Rabbit anti-MUC4 antibody (ET1705-13) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1705-13, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 542 kDa
Observed band size: 100-170 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1705-13, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Ovarian serous carcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1705-13, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1705-13, 1/100, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Pancreas
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET1705-13, 1/100, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative expression of MUC4 protein in pancreas is consistent with the predicted expression pattern.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"