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Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]

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Specification

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Catalog# ET1705-19

Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Calretinin aa 60-100.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 32 kDa

Positive Control

Mouse brain tissue lysate, rat brain tissue lysate, mouse hippocampus tissue lysate, rat hippocampus tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.

Conjugation

unconjugated

Clone Number

JM12-93

RRID

AB_3070551

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:5,000

  • IHC-Fr

  • 1:500

  • IF-Tissue

  • 1:1,000

Target

Function

Calretinin, also known as calbindin 2 (formerly 29 kDa calbindin), is a calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. Calretinin is abundantly expressed in neurons including retina (which gave it the name) and cortical interneurons. Expression was found in different neurons than that of the similar vitamin D-dependent calcium-binding protein, calbindin-28kDa. Calretinin has an important role as a modulator of neuronal excitability including the induction of long-term potentiation. Loss of expression of calretinin in hippocampal interneurons has been suggested to be relevant in temporal lobe epilepsy. It is expressed in a number of other locations including hair follicles.

Background References

1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).

2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).

Sequence Similarity

Belongs to the calbindin family.

Tissue Specificity

Brain.

Subcellular Location

Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.

UNIPROT

SwissProt: P22676 Human

SwissProt: Q08331 Mouse

SwissProt: P47728 Rat

Synonyms

29 kDa calbindin antibody

CAB 29 antibody

CAB29 antibody

CAL 2 antibody

CAL2 antibody

CALB 2 antibody

CALB2 antibody

CALB2_HUMAN antibody

Calbindin 2 29kDa antibody

Calbindin 2 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Calretinin antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Calretinin antibody (ET1705-19) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Calretinin antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Calretinin antibody (ET1705-19) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Calretinin antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Calretinin antibody (ET1705-19) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Calretinin on different lysates with Rabbit anti-Calretinin antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br />Lane 3: Mouse hippocampus tissue lysate<br />Lane 4: Rat hippocampus tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 7 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-19" style="font-weight: bold;text-decoration: underline;">ET1705-19</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Calretinin on different lysates with Rabbit anti-Calretinin antibody (ET1705-19) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: Mouse hippocampus tissue lysate
    Lane 4: Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 27 kDa

    Exposure time: 7 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-19) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

iFluor™ 488 Conjugated Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]

Application: IF-Cell,FC

Reactivity: Human

Conjugate: iFluor™ 488