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PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17]

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Specification

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Catalog# ET1705-4

PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PKC mu Recombinant Rabbit Monoclonal Antibody [JM30-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PKC aa 201-250 / 912.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 102 kDa

Positive Control

Hela cell lysate, mouse kidney tissue lysate, PC-12 cell lysate, PC-3 cell lysates.

Conjugation

unconjugated

Clone Number

JM30-17

RRID

AB_3070568

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response.

Background References

1. Sehgal L et al. 14-3-3-Mediated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo. J Cell Sci 127:2174-88 (2014).

2. Sinnett-Smith J et al. Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts. J Biol Chem 286:511-20 (2011).

Sequence Similarity

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Post-translational Modification

Phosphorylated at Ser-397 and Ser-401 by MAPK13 during regulation of insulin secretion in pancreatic beta cells. Phosphorylated by DAPK1. Phosphorylated at Tyr-95 and by ABL at Tyr-463, which primes the kinase in response to oxidative stress, and promotes a second step activating phosphorylation at Ser-738/Ser-742 by PKRD. Phosphorylated on Ser-910 upon S.enterica infection in macrophages (By similarity).

Subcellular Location

Cell membrane, trans-Golgi network, Cytoplasm.

UNIPROT

SwissProt: Q15139 Human

SwissProt: Q62101 Mouse

SwissProt: Q9WTQ1 Rat

Synonyms

KPCD1_HUMAN antibody

nPKC D1 antibody

nPKC mu antibody

nPKC-D1 antibody

nPKC-mu antibody

nPKCD1 antibody

nPKCmu antibody

PKC antibody

PKC MU antibody

PKCM antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PKC mu on different lysates with Rabbit anti-PKC mu antibody (<a href="/products/ET1705-4" style="font-weight: bold;text-decoration: underline;">ET1705-4</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si PKC mu cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 102 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-4" style="font-weight: bold;text-decoration: underline;">ET1705-4</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PKC mu on different lysates with Rabbit anti-PKC mu antibody (ET1705-4) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si PKC mu cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PKC mu on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-4" style="font-weight: bold;text-decoration: underline;">ET1705-4</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Hela cell lysate<br />Lane 2: Mouse kidney tissue lysate<br />Lane 3: PC-12 cell lysate

    Western blot analysis of PKC mu on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: Mouse kidney tissue lysate
    Lane 3: PC-12 cell lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation