SKP1 Recombinant Rabbit Monoclonal Antibody [JM27-37]
Catalog# ET1705-43
SKP1 Recombinant Rabbit Monoclonal Antibody [JM27-37]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Rat
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Mouse
Predicted species support after-sales service
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unconjugated
Overview
Product Name
SKP1 Recombinant Rabbit Monoclonal Antibody [JM27-37]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Skp1 aa 106-155 / 163.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Molecular Weight
Predicted band size: 19 kDa
Positive Control
PC-12 cell lysates, Hela, SH-SY5Y, human breast tissue.
Conjugation
unconjugated
Clone Number
JM27-37
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:100
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
S-phase kinase-associated protein 1 is an enzyme that in humans is encoded by the SKP1 gene. This gene encodes a protein that is a member of the SCF ubiquitin ligase protein complex. It binds to F-box proteins (proteins containing an F-box motif), such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis. The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
Background References
1. Kainulainen M et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014).
2. Wang D et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 109:9493-8 (2012).
Sequence Similarity
Belongs to the SKP1 family.
Post-translational Modification
Undergoes autophagy-mediated degradation in the liver in a time-dependent manner.
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
Cyclin A/CDK2 associated p19 antibody
Cyclin A/CDK2 associated protein p19 antibody
Cyclin-A/CDK2-associated protein p19 antibody
EMC19 antibody
MGC34403 antibody
OCP 2 antibody
OCP II antibody
OCP II protein antibody
OCP-2 antibody
OCP-II antibody
ExpandImages
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Western blot analysis of SKP1 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
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ICC staining of SKP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of SKP1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SKP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of SKP1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-43, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"