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c-Rel Recombinant Rabbit Monoclonal Antibody [JM72-93]

Usd: 385

Specification

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Catalog# ET1705-44

c-Rel Recombinant Rabbit Monoclonal Antibody [JM72-93]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

c-Rel Recombinant Rabbit Monoclonal Antibody [JM72-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human c-Rel aa 501-550 / 619.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 69 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, Raji cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate, U-937 cell lysate, human tonsil tissue, human colon cancer tissue, human skin tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

JM72-93

RRID

AB_3070571

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

Target

Function

c-Rel is the cellular cognate of v-Rel, the avian reticuloendotheliosis virus strain T transforming gene. v-Rel encodes a phosphoprotein that is located in the cytoplasm of transformed spleen cells and in the nucleus of non-transformed fibroblasts, in contrast to the c-Rel protein, which is cytoplasmic. c-Rel has been shown to represent a constituent of the κB site binding transcription factor NFκB, which plays a crucial role in the expression of immunoglobulin κ light chain gene. c-Rel is a myeloid checkpoint protein that can be targeted for treating cancer. c-Rel has an important role in B-cell survival and proliferation. The REL gene is amplified or mutated in several human B-cell lymphomas, including diffuse large B-cell lymphoma and Hodgkin's lymphoma.

Background References

1. Haljasorg, U. et al. A highly conserved NF-κB-responsive enhancer is critical for thymic expression of Aire in mice. European journal of immunology. 45: 3246-56 (2015).

2. Hodgson, A. et al. Metalloprotease NleC Suppresses Host NF-κB/Inflammatory Responses by Cleaving p65 and Interfering with the p65/RPS3 Interaction. PLoS pathogens 11(3): e1004705 (2015).

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: Q04864 Human

Synonyms

Avian reticuloendotheliosis antibody

C REL antibody

C Rel protein antibody

c Rel proto oncogene protein antibody

Oncogene REL antibody

Oncogene REL avian reticuloendotheliosis antibody

Proto-oncogene c-Rel antibody

REL antibody

REL_HUMAN antibody

v rel avian reticuloendotheliosis viral oncogene homolog antibody

Expand

Images

  • Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (<a href="/products/ET1705-44" style="font-weight: bold;text-decoration: underline;">ET1705-44</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: Raji cell lysate<br />Lane 4: Daudi cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: A431 cell lysate<br />Lane 7: U-937 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 69 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-44" style="font-weight: bold;text-decoration: underline;">ET1705-44</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: Raji cell lysate
    Lane 4: Daudi cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: A431 cell lysate
    Lane 7: U-937 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 69 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (<a href="/products/ET1705-44" style="font-weight: bold;text-decoration: underline;">ET1705-44</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-c-Rel KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 69 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 1 minute 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-44" style="font-weight: bold;text-decoration: underline;">ET1705-44</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of c-Rel on different lysates with Rabbit anti-c-Rel antibody (ET1705-44) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-c-Rel KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 69 kDa
    Observed band size: 75 kDa

    Exposure time: 1 minute 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-44) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Rel antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"