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ENO1 Recombinant Rabbit Monoclonal Antibody [JM63-53] (ET1705-56)

Price: Regular price $385.00

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Catalog# ET1705-56

Rabbit Monoclonal to ENO1

Tested for

  • WB

  • FC

  • IF-Tissue

  • IHC-P

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

  • Zebrafish

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ENO1 Recombinant Rabbit Monoclonal Antibody [JM63-53] (ET1705-56)

Immunogen

Synthetic peptide within human eno1 aa 1-50 / 434.

Host

Rabbit

Positive Control

HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Zebrafish tissue lysate, human lung cancer tissue, HeLa, NIH/3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM63-53

RRID

AB_3070584

APPLICATION DILUTION

  • WB

  • 1:2,000

  • FC

  • 1:1,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 47 kDa

Isotype

IgG

TARGET

UNIPROT #

P06733

PROTEIN NAME

ENO1

SYNONYMS

2 phospho D glycerate hydro lyase antibody;2-phospho-D-glycerate hydro-lyase antibody;Alpha enolase antibody;Alpha enolase like 1 antibody;Alpha-enolase antibody;C myc promoter binding protein antibody;C-myc promoter-binding protein antibody;EC 4.2.1.11 antibody;eno1 antibody;ENO1L1 antibody;ENOA_HUMAN antibody;Enolase 1 (alpha) antibody;Enolase 1 (alpha) like 1 antibody;Enolase 1 antibody;Enolase alpha antibody;MBP 1 antibody;MBP-1 antibody;MBP1 antibody;MBPB1 antibody;MPB 1 antibody;MPB-1 antibody;MPB1 antibody;MYC promoter binding protein 1 antibody;NNE antibody;Non neural enolase antibody;Non-neural enolase antibody;Phosphopyruvate hydratase antibody;Plasminogen binding protein antibody;Plasminogen-binding protein antibody;PPH antibody;Tau crystallin antibody

SEQUENCE SIMILARITIES

Belongs to the enolase family.

TISSUE SPECIFICITY

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

DEVELOPMENTAL STAGE

During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

SUBCELLULAR LOCATION

Cell membrane, Cytoplasm, Membrane, Nucleus.

FUNCTION

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

CITATIONS

  • Tang, Y., Jin, Y. H., Li, H. L., Xin, H., Chen, J. D., Li, X. Y., & Pan, Y. F.

    PBRM1 deficiency oncogenic addiction is associated with activated AKT–mTOR signalling and aerobic glycolysis in clear cell renal cell carcinoma cells

  • He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S.

    PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis

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