cbx7 Recombinant Rabbit Monoclonal Antibody [JM44-63]
Catalog# ET1705-67
cbx7 Recombinant Rabbit Monoclonal Antibody [JM44-63]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
cbx7 Recombinant Rabbit Monoclonal Antibody [JM44-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human cbx7 aa 1-190 / 251.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 28 kDa
Positive Control
A431 cell lysates, Hela.
Conjugation
unconjugated
Clone Number
JM44-63
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
CBX7 (Chromobox protein homolog 7) is a 251 amino acid nuclear protein that contains one N-terminal chromo domain and one C-terminal Pc box. Highly expressed in kidney, brain, heart and skeletal muscle, with weaker expression in peripheral blood leukocytes, CBX7 functions as a component of the chromatin-associated polycomb complex (PcG) and is involved in maintaining the transcriptionally repressed state of target genes. Additionally, CBX7 modifies chromatin and is thought to extend the cellular life span of epithelial cells by repressing p14 ARF expression, while simultaneously repressing telomerase activity. Due to its ability to repress the transcription of cell-cycle related proteins, CBX7 is thought to play a role in tumorigenesis, specifically in the development of follicular lymphoma and thyroid cancer.
Background References
1. Connelly KE et al. CBX Chromodomain Inhibition Enhances Chemotherapy Response in Glioblastoma Multiforme. Yale J Biol Med 89:431-440 (2016).
2. Zheng X et al. Critical evaluation of Cbx7 downregulation in primary colon carcinomas and its clinical significance in Chinese patients. BMC Cancer 15:145 (2015).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Cbx 7 antibody
CBX7 antibody
Chromobox homolog 7 antibody
Chromobox protein homolog 7 antibody
Images
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Western blot analysis of cbx7 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of cbx7 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-67, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"