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DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]

Usd: 385

Specification

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Catalog# ET1705-71

DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]

Application

  • WB

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DDAH1 aa 100-285 / 285.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 31 kDa

Positive Control

SH-SY5Y cell lysate, HepG2 cell lysate, HUVEC cell lysate, human prostate tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

JM53-18

RRID

AB_3070601

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.

Background References

1. Wang H et al. DDAH1 plays dual roles in PM2.5 induced cell death in A549 cells. Biochim Biophys Acta 1860:2793-801 (2016).

2. Jung CS et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014).

Sequence Similarity

Belongs to the DDAH family.

Tissue Specificity

Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O94760 Human

Synonyms

DDAH antibody

DDAH I antibody

DDAH-1 antibody

DDAH1 antibody

DDAH1_HUMAN antibody

DDAHI antibody

Dimethylargininase 1 antibody

Dimethylargininase-1 antibody

Dimethylarginine dimethylaminohydrolase 1 antibody

N(G) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (<a href="/products/ET1705-71" style="font-weight: bold;text-decoration: underline;">ET1705-71</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-DDAH1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-71" style="font-weight: bold;text-decoration: underline;">ET1705-71</a>) at 1/5,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-DDAH1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 37 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (<a href="/products/ET1705-71" style="font-weight: bold;text-decoration: underline;">ET1705-71</a>) at 1/5,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: HUVEC cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-71" style="font-weight: bold;text-decoration: underline;">ET1705-71</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HUVEC cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 37 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"