DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
Catalog# ET1705-77
DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
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WB
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IHC-P
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IF-Cell
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Human
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unconjugated
Overview
Product Name
DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DNA Ligase IV aa 484-705 / 911.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 104 kDa
Positive Control
HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, human colon carcinoma tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
JM64-32
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:10-1:50
Target
Function
The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.
Background References
1. Rasmussen RD et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol 10(5):751-63 (2016).
2. Morgenroth A et al. Hedgehog signaling sensitizes glioma stem cells to endogenous nano-irradiation. Oncotarget 5:5483-93 (2014).
Sequence Similarity
Belongs to the ATP-dependent DNA ligase family.
Tissue Specificity
Testis, thymus, prostate and heart.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
DNA ligase IV ATP dependent antibody
DNA joinase antibody
DNA ligase 4 antibody
DNA ligase IV antibody
DNA repair enzyme antibody
DNLI4_HUMAN antibody
LIG 4 antibody
LIG4 antibody
LIG4S antibody
Ligase IV antibody
ExpandImages
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Western blot analysis of DNA Ligase IV on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"