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DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]

Usd: 385

Specification

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Catalog# ET1705-77

DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human DNA Ligase IV aa 484-705 / 911.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell

Target Molecular Weight

Predicted band size: 104 kDa

Positive Control

Jurkat cell lysate, human colon carcinoma tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

JM64-32

RRID

AB_3073473

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • IF-Cell

  • 1:10-1:50

Target

Function

The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.

Background References

1. Rasmussen RD et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol 10(5):751-63 (2016).

2. Morgenroth A et al. Hedgehog signaling sensitizes glioma stem cells to endogenous nano-irradiation. Oncotarget 5:5483-93 (2014).

Sequence Similarity

Belongs to the ATP-dependent DNA ligase family.

Tissue Specificity

Testis, thymus, prostate and heart.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P49917 Human

Synonyms

DNA ligase IV ATP dependent antibody

DNA joinase antibody

DNA ligase 4 antibody

DNA ligase IV antibody

DNA repair enzyme antibody

DNLI4_HUMAN antibody

LIG 4 antibody

LIG4 antibody

LIG4S antibody

Ligase IV antibody

Expand

Images

  • Western blot analysis of DNA Ligase IV on Jurkat cell lysate with Rabbit anti-DNA Ligase IV antibody (<a href="/products/ET1705-77" style="font-weight: bold;text-decoration: underline;">ET1705-77</a>) at 1:500 dilution.<br /><br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 2 minutes; ECL: K1802<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1705-77" style="font-weight: bold;text-decoration: underline;">ET1705-77</a>, 1/500 in 5% BSA, room temperature for 2 hours<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 104 kDa<br />Observed band size: 104 kDa

    Western blot analysis of DNA Ligase IV on Jurkat cell lysate with Rabbit anti-DNA Ligase IV antibody (ET1705-77) at 1:500 dilution.


    Lysates/proteins at 10 µg/Lane.
    Exposure time: 2 minutes; ECL: K1802


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1705-77, 1/500 in 5% BSA, room temperature for 2 hours
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 104 kDa
    Observed band size: 104 kDa

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-77" style="font-weight: bold;text-decoration: underline;">ET1705-77</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-77" style="font-weight: bold;text-decoration: underline;">ET1705-77</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"