Dynein intermediate chain 1 Recombinant Rabbit Monoclonal Antibody [JM11-38]
Catalog# ET1705-8
Dynein intermediate chain 1 Recombinant Rabbit Monoclonal Antibody [JM11-38]
-
WB
-
IHC-P
-
Mouse
-
Human
-
Rat
Predicted species support after-sales service
-
unconjugated
Overview
Product Name
Dynein intermediate chain 1 Recombinant Rabbit Monoclonal Antibody [JM11-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Dynein intermediate chain 1 aa 1-240 / 699.
Species Reactivity
Mouse (Predicted: Human, Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 79 kDa
Positive Control
Mouse fallopian tube tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
JM11-38
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
Target
Function
Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. Axonemal Dynein motors contain one to three non-identical heavy chains and cause a sliding of microtubules in the axonemes of cilia and flagella in a mechanism necessary for cilia to beat and propel the cell. Cytoplasmic Dyneins, such as Dynein IC1, cytosolic and Dynein IC2, cytosolic, comprise an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. This complex performs functions necessary for cell survival, such as organelle transport and centrosome assembly. The carboxy terminus of Dynein is important for microtubule-dependent motility and is highly conserved, while the amino terminal regions are more variable. Several proteins regulate Dynein activity, including dynactin, LIS1 and NudEL(NudE-like).
Background References
1. Mahale SP et al. Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint. PLoS One 11:e0159646 (2016).
2. Pillai S et al. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun 6:10072 (2015).
Sequence Similarity
Belongs to the dynein intermediate chain family.
Subcellular Location
Cell projection, Cilium, Cytoplasm, Cytoskeleton, Dynein, Microtubule.
Synonyms
Axonemal dynein intermediate chain 1 antibody
Axonemal dynein intermediate chain 2 antibody
CILD 1 antibody
CILD1 antibody
Cytoplasmic dynein 1 intermediate chain 1 antibody
Cytoplasmic dynein 1 intermediate chain 2 antibody
Cytoplasmic dynein intermediate chain 1 antibody
Cytoplasmic dynein intermediate chain 2 antibody
DH IC 1 antibody
DH IC 2 antibody
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-Dynein intermediate chain 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dynein intermediate chain 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"