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Klotho Recombinant Rabbit Monoclonal Antibody [JM93-76]

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Specification

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Catalog# ET1705-88

Klotho Recombinant Rabbit Monoclonal Antibody [JM93-76]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Klotho Recombinant Rabbit Monoclonal Antibody [JM93-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Klotho aa 404-453 / 1,012.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 116 kDa

Positive Control

Human kidney tissue lysates, Human serum lysate, human kidney tissue lysate, human kidney tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

JM93-76

RRID

AB_3070616

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:5,000

  • IHC-P

  • 1:50-1:200

Target

Function

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Background References

1. Mazzotta C et al. Proangiogenic effects of soluble a-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther 19:27 (2017).

2. Sun J et al. Astragaloside IV protects new born rats from anesthesia-induced apoptosis in the developing brain. Exp Ther Med 12:1829-1835 (2016).

Sequence Similarity

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Tissue Specificity

Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.

Post-translational Modification

N-glycosylated.

Subcellular Location

Cell membrane. Apical cell membrane. Secreted.

UNIPROT

SwissProt: Q9UEF7 Human

SwissProt: O35082 Mouse

Synonyms

Kl antibody

KLOT_HUMAN antibody

Klotho peptide antibody

Images

  • Western blot analysis of Klotho on human kidney tissue lysates with Rabbit anti-Klotho antibody (<a href="/products/ET1705-88" style="font-weight: bold;text-decoration: underline;">ET1705-88</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 116 kDa<br />Observed band size: 61 kDa<br /><br />Exposure time: 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-88" style="font-weight: bold;text-decoration: underline;">ET1705-88</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Klotho on human kidney tissue lysates with Rabbit anti-Klotho antibody (ET1705-88) at 1/5,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 116 kDa
    Observed band size: 61 kDa

    Exposure time: 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-88) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Klotho antibody (<a href="/products/ET1705-88" style="font-weight: bold;text-decoration: underline;">ET1705-88</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-88" style="font-weight: bold;text-decoration: underline;">ET1705-88</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Klotho antibody (ET1705-88) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-88) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Klotho antibody (<a href="/products/ET1705-88" style="font-weight: bold;text-decoration: underline;">ET1705-88</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-88" style="font-weight: bold;text-decoration: underline;">ET1705-88</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Klotho antibody (ET1705-88) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-88) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation