Eg5 Recombinant Rabbit Monoclonal Antibody [JU83-16]

Function

Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.

Background References

1. Chien JY et al. aTAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov 2:16006 (2016).

2. Drosopoulos K et al. APC/C is an essential regulator of centrosome clustering. Nat Commun 5:3686 (2014).

Sequence Similarity

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

Post-translational Modification

Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).; A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.

Subcellular Location

Cytoplasm, Cytoskeleton, Microtubule.

UNIPROT

SwissProt: P52732 Human

Synonyms