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GluR2 Recombinant Rabbit Monoclonal Antibody [JU20-43]

Usd: 385

Specification

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Catalog# ET1706-06

GluR2 Recombinant Rabbit Monoclonal Antibody [JU20-43]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GluR2 Recombinant Rabbit Monoclonal Antibody [JU20-43]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human GluR2 aa 181-230 / 883.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 99 kDa

Positive Control

Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, N2A, SH-SY5Y, rat brain tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

JU20-43

RRID

AB_3070632

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Lonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Background References

1. Lam RS et al. Functional Maturation of Human Stem Cell-Derived Neurons in Long-Term Cultures. PLoS One 12:e0169506 (2017).

2. Li WG et al. ASIC1a regulates insular long-term depression and is required for the extinction of conditioned taste aversion. Nat Commun 7:13770 (2016).

Sequence Similarity

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.

Post-translational Modification

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).; Phosphorylation at Tyr-876 is required forc interaction with IQSEC1 and ARF6 activation.

Subcellular Location

Cell membrane, postsynaptic cell membrane, postsynaptic density membrane, endoplasmic reticulum membrane.

UNIPROT

SwissProt: P42262 Human

SwissProt: P23819 Mouse

SwissProt: P19491 Rat

Synonyms

AMPA 2 antibody

AMPA selective glutamate receptor 2 antibody

AMPA-selective glutamate receptor 2 antibody

AMPA2 antibody

GluA2 antibody

GLUR 2 antibody

GLUR B antibody

GluR K2 antibody

GluR-2 antibody

GluR-B antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of GluR2 on different lysates with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (40 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 3: Mouse heart tissue lysate (negative) (40 µg/Lane)<br />Lane 4: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 99 kDa<br />Observed band size: 99 kDa<br /><br />Exposure time: Lane 1: 3 minutes; Lane 3: 46 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of GluR2 on different lysates with Rabbit anti-GluR2 antibody (ET1706-06) at 1/2,000 dilution.

    Lane 1: Human brain tissue lysate (40 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)
    Lane 3: Mouse heart tissue lysate (negative) (40 µg/Lane)
    Lane 4: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 99 kDa
    Observed band size: 99 kDa

    Exposure time: Lane 1: 3 minutes; Lane 3: 46 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of N2A cells labeling GluR2 with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of N2A cells labeling GluR2 with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling GluR2 with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY5Y cells labeling GluR2 with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody (ET1706-06) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-06" style="font-weight: bold;text-decoration: underline;">ET1706-06</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody (ET1706-06) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation