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USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]

Usd: 385

Specification

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Catalog# ET1706-14

USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human USP13 aa 739-788 / 863.

Species Reactivity

Human, Mouse

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 97 kDa

Positive Control

SH-SY5Y cell lysates, mouse testis tissue lysates.

Conjugation

unconjugated

Clone Number

JU72-38

RRID

AB_3070639

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IP

  • 1:10-1:50

Target

Function

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.

Background References

1. Esquerdo M et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016).

2. Geng J et al. Down-regulation of USP13 mediates phenotype transformation of fibroblasts in idiopathic pulmonary fibrosis. Respir Res 16:124 (2015).

Sequence Similarity

Belongs to the peptidase C19 family.

Tissue Specificity

Highly expressed in ovary and testes.

Subcellular Location

Nucleus. Cytoplasm.

UNIPROT

SwissProt: Q92995 Human

SwissProt: Q5BKP2 Mouse

Synonyms

Deubiquitinating enzyme 13 antibody

Isopeptidase T 3 antibody

Isopeptidase T-3 antibody

Isopeptidase T3 antibody

ISOT 3 antibody

ISOT-3 antibody

ISOT3 antibody

Ubiquitin carboxyl terminal hydrolase 13 antibody

Ubiquitin carboxyl-terminal hydrolase 13 antibody

Ubiquitin specific peptidase 13 (isopeptidase T3) antibody

Expand

Images

  • Western blot analysis of USP13 on SH-SY5Y cell lysates with Rabbit anti-USP13 antibody (<a href="/products/ET1706-14" style="font-weight: bold;text-decoration: underline;">ET1706-14</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 97 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-14" style="font-weight: bold;text-decoration: underline;">ET1706-14</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of USP13 on SH-SY5Y cell lysates with Rabbit anti-USP13 antibody (ET1706-14) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 97 kDa
    Observed band size: 100 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of USP13 on mouse testis tissue lysates with Rabbit anti-USP13 antibody (<a href="/products/ET1706-14" style="font-weight: bold;text-decoration: underline;">ET1706-14</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-14" style="font-weight: bold;text-decoration: underline;">ET1706-14</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of USP13 on mouse testis tissue lysates with Rabbit anti-USP13 antibody (ET1706-14) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-14) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation