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SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33]

Usd: 385

Specification

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Catalog# ET1706-30

SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SDHB aa 201-250 / 280.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 32 kDa

Positive Control

HepG2 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Rat liver tissue lysate, Human liver tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, human liver tissue, human colon carcinoma tissue.

Conjugation

unconjugated

Clone Number

JU02-33

RRID

AB_3073475

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

Target

Function

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

Background References

1. Rohlenova K et al. Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2(high) Breast Cancer. Antioxid Redox Signal 26:84-103 (2017).

2. Stram AR et al. Progressive mitochondrial protein lysine acetylation and heart failure in a model of Friedreich\'s ataxia cardiomyopathy. PLoS One 12:e0178354 (2017).

Sequence Similarity

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

UNIPROT

SwissProt: P21912 Human

SwissProt: Q9CQA3 Mouse

SwissProt: P21913 Rat

Synonyms

CWS2 antibody

DHSB_HUMAN antibody

FLJ92337 antibody Ip antibody

Iron sulfur subunit antibody

Iron sulfur subunit of complex II antibody

Iron-sulfur subunit of complex II antibody

mitochondrial antibody

PGL 4 antibody

PGL4 antibody

SDH 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of SDHB with anti-SDHB antibody [JU02-33] (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1:1,000 dilution.<br /><br />Lane 1: Wild-type Hela whole cell lysate.<br />Lane 2: SDHB knockout Hela whole cell lysate.<br /><br /><a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a> was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>, 1/1,000) and Anti-GAPDH antibody (<a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><br />Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

    ☑ Knockout (KO)

    All lanes: Western blot analysis of SDHB with anti-SDHB antibody [JU02-33] (ET1706-30) at 1:1,000 dilution.

    Lane 1: Wild-type Hela whole cell lysate.
    Lane 2: SDHB knockout Hela whole cell lysate.

    ET1706-30 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ET1706-30, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

  • Western blot analysis of SDHB on different lysates with Rabbit anti-SDHB antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 3: Rat spleen tissue lysate (40 µg/Lane)<br />Lane 4: Rat liver tissue lysate (40 µg/Lane)<br />Lane 5: Human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 32 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 24 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SDHB on different lysates with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 3: Rat spleen tissue lysate (40 µg/Lane)
    Lane 4: Rat liver tissue lysate (40 µg/Lane)
    Lane 5: Human liver tissue lysate (40 µg/Lane)

    Predicted band size: 32 kDa
    Observed band size: 27 kDa

    Exposure time: 24 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-30) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SDHB antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-30) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SDHB antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-30) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SDHB antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-30" style="font-weight: bold;text-decoration: underline;">ET1706-30</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-30) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation