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Wnt5a Recombinant Rabbit Monoclonal Antibody [JU30-30]

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Specification

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Catalog# ET1706-33

Wnt5a Recombinant Rabbit Monoclonal Antibody [JU30-30]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Wnt5a Recombinant Rabbit Monoclonal Antibody [JU30-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Wnt5a aa 331-380 / 380.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 42 kDa

Positive Control

HeLa cell lysates, human lung cancer tissue.

Conjugation

unconjugated

Clone Number

JU30-30

RRID

AB_3070654

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:1,000

Target

Function

The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene "wingless" and to its vertebrate ortholog, Int1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Northern blot analysis detects expression of Wnt-5a in brain, lung, and heart. At least five distinct Wnt-5a transcripts are observed, which are due to transcript variability 5-prime to the initiation methionine. In situ hybridization detects a complex spatial and temporal pattern of Wnt-5a in the mouse, including expression in the developing central nervous system, limbs, facial processes, and the posterior region of the fetus. Human frizzled-5 is the receptor for the Wnt-5a ligand. It is suggested that Wnt-5a augments primitive hematopoietic development in vivo and represents an in vivo regulator of hematopoietic stem cell function in the human.

Background References

1. Kremenevskaja N et al. Wnt-5a has tumor suppressor activity in thyroid carcinoma. Oncogene 24:2144-2154 (2005).

2. Dissanayake SK et al. The Wnt5A/protein kinase C pathway mediates motility in melanoma cells via the inhibition of metastasis suppressors and initiation of an epithelial to mesenchymal transition. J. Biol. Chem. 282:17259-17271 (2007).

Sequence Similarity

Belongs to the Wnt family.

Tissue Specificity

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Detected in neonate heart and lung.

Post-translational Modification

Glycosylation is necessary for secretion but not for activity.; Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.; Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P41221 Human

Synonyms

hWNT 5A antibody

hWNT5A antibody

Protein Wnt 5a antibody

Protein Wnt-5a antibody

Protein Wnt5a antibody

Wingless type MMTV integration site family member 5A antibody

Wnt 5a antibody

WNT 5A protein antibody

WNT 5A protein precursor antibody

WNT5A antibody

Expand

Images

  • Western blot analysis of Wnt5a on HeLa cell lysates with Rabbit anti-Wnt5a antibody (<a href="/products/ET1706-33" style="font-weight: bold;text-decoration: underline;">ET1706-33</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-33" style="font-weight: bold;text-decoration: underline;">ET1706-33</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Wnt5a on HeLa cell lysates with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 45 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-33) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Wnt5a antibody (<a href="/products/ET1706-33" style="font-weight: bold;text-decoration: underline;">ET1706-33</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-33" style="font-weight: bold;text-decoration: underline;">ET1706-33</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-33) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation