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Staufen Recombinant Rabbit Monoclonal Antibody [JU30-85]

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Specification

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Catalog# ET1706-41

Staufen Recombinant Rabbit Monoclonal Antibody [JU30-85]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Staufen Recombinant Rabbit Monoclonal Antibody [JU30-85]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Staufen aa 528-577 / 577.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, IF-Tissue, FC

Target Molecular Weight

Predicted band size: 63 kDa

Positive Control

K562 cell lysates, LOVO cell lysates, HepG2, LOVO, SH-SY5Y

Conjugation

unconjugated

Clone Number

JU30-85

RRID

AB_3070661

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long.

Background References

1. Crawford Parks TE et al. Novel Roles for Staufen1 in Embryonal and Alveolar Rhabdomyosarcoma via c-myc-dependent and -independent events. Sci Rep 7:42342 (2017).

2. Kobayashi S et al. Local Somatodendritic Translation and Hyperphosphorylation of Tau Protein Triggered by AMPA and NMDA Receptor Stimulation. EBioMedicine 20:120-126 (2017).

Tissue Specificity

Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.

Subcellular Location

Cytoplasm. Rough endoplasmic reticulum.

UNIPROT

SwissProt: O95793 Human

SwissProt: Q9Z108 Mouse

Entrez Gene: 84496 Rat

Synonyms

Double stranded RNA binding protein Staufen homolog 1 antibody

Double stranded RNA binding protein Staufen homolog antibody

Double-stranded RNA-binding protein Staufen homolog 1 antibody

FLJ25010 antibody

MGC124588 antibody

PPP1R150 antibody

STAU antibody

STAU1 antibody

STAU1_HUMAN antibody

staufen antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Staufen on different lysates with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Staufen KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63/55 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Staufen on different lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Staufen KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63/55 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Staufen on K562 cell lysates with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Staufen on K562 cell lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 53 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Staufen on LOVO cell lysates with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Staufen on LOVO cell lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 53 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HepG2 cells labeling Staufen with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HepG2 cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of LOVO cells labeling Staufen with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of LOVO cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling Staufen with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY5Y cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of Staufen was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1706-41" style="font-weight: bold;text-decoration: underline;">ET1706-41</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Staufen was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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