ATG16L1 Recombinant Rabbit Monoclonal Antibody [JU30-62] (ET7106-65)
Catalog# ET7106-65
Rabbit Monoclonal to ATG16L1
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68/66 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ATG16L1 Recombinant Rabbit Monoclonal Antibody [JU30-62] (ET7106-65)
Immunogen
Recombinant protein within n-terminal human atg16l1.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, mouse colon tissue, PC-12 cell lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU30-62
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ATG16L1
SYNONYMS
A16L1_HUMAN antibody;APG16 like 1 antibody;APG16-like 1 antibody;APG16L antibody;APG16L beta antibody;ATG16 autophagy related 16 like 1 antibody;ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody;ATG16A antibody;ATG16L antibody;Atg16l1 antibody;Autophagy related protein 16 1 antibody;Autophagy-related protein 16-1 antibody;FLJ00045 antibody;FLJ10035 antibody;FLJ10828 antibody;FLJ22677 antibody;IBD10 antibody;OTTHUMP00000164391 antibody;OTTHUMP00000164393 antibody;OTTHUMP00000165876 antibody;OTTHUMP00000165877 antibody;WD repeat domain 30 antibody;WDR30 antibody
SEQUENCE SIMILARITIES
Belongs to the WD repeat ATG16 family.
POST-TRANSLATIONAL MODIFICATION
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.; Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production. Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response. Plays a role in regulating morphology and function of Paneth cell.
CITATIONS
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Yin, L., Lv, M., Qiu, X., Wang, X., Zhang, A., Yang, K., & Zhou, H.
IFN-γ Manipulates NOD1-Mediated Interaction of Autophagy and Edwardsiella piscicida to Augment Intracellular Clearance in Fish
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WB
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Grass carp
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Citation
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