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BDKRB2 Recombinant Rabbit Monoclonal Antibody [JU39-04]

Usd: 385

Specification

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Catalog# ET7106-74

BDKRB2 Recombinant Rabbit Monoclonal Antibody [JU39-04]

Application

  • WB

  • FC

  • IP

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BDKRB2 Recombinant Rabbit Monoclonal Antibody [JU39-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human BDKRB2 aa 262-391 / 391.

Species Reactivity

Human, Mouse

Validated Applications

WB, FC, IP, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 44 kDa

Positive Control

Neuro-2a cell lysates, MCF-7 cell lysates, mouse brain tissue, Neuro-2a, SH-SY5Y.

Conjugation

unconjugated

Clone Number

JU39-04

RRID

AB_3070694

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:1:1,000

  • IP

  • 1-2μg/sample

  • IHC-P

  • 1:200

  • IF-Cell

  • 1:100

Target

Function

Kinins are important biologically active peptides that mediate cardiovascular homeostasis, inflammation and nociception. Bradykinin, the major effector peptide of the kallikrein-kinin system, is regulated by angiotensin-converting enzyme (ACE), which degrades the peptide. Bradykinin normally exerts its effects through the activation of two seven transmembrane G-protein coupled receptors, named B1 and B2. The B2 receptor is constitutively expressed and preferentially binds full length bradykinin. Deletion of the B2 receptor leads to salt-sensitive hypertension and altered nociception in mice. The B1 receptor binds to derivatives of bradykinin and kallidin, which are produced by carboxypeptidase action to generate the products des-Arg9-bradykinin and des-Arg10-kallidin, respectively. The expression of the B1 receptor is inducible by inflammatory mediators, such as bacterial lipopolysaccharide (LPS) and cytokines. The B1 and B2 receptors represent potential therapeutic targets for treatment of inflammatory disorders and cardiovascular diseases.

Background References

1. Blaukat A et al. Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function. J Biol Chem 276:40431-40440 (2001).

2. Ma J-X et al. Structure and chromosomal localization of the gene (BDKRB2) encoding human bradykinin B2 receptor. Genomics 23:362-369 (1994).

Sequence Similarity

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Tissue Specificity

Ubiquitous. Widespread in normal smooth muscle tissue and neurons.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P30411 Human

SwissProt:P32299 Mouse

Synonyms

B2 antibody

B2 bradykinin receptor antibody

B2BKR antibody

B2BRA antibody

B2R antibody

BDKR B2 antibody

BDKRB 2 antibody

BDKRB2 antibody

BK 2 antibody

BK 2 receptor antibody

Expand

Images

  • Western blot analysis of BDKRB2 on Neuro-2a cell lysates with Rabbit anti-BDKRB2 antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>) at 1/2,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 80-90 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BDKRB2 on Neuro-2a cell lysates with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/2,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 80-90 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of BDKRB2 on MCF-7 cell lysate using anti-BDKRB2 antibody at 1/500 dilution.

    Western blot analysis of BDKRB2 on MCF-7 cell lysate using anti-BDKRB2 antibody at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-BDKRB2 antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-74) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of Neuro-2a cells labeling BDKRB2 with Rabbit anti-BDKRB2 antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BDKRB2 antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Neuro-2a cells labeling BDKRB2 with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of Neuro-2a cells labeling BDKRB2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Neuro-2a cells labeling BDKRB2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET7106-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • BDKRB2 was immunoprecipitated from 0.2 mg Neuro-2a cell lysate with <a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a> at 1/2,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Neuro-2a cell lysate (input)<br />Lane 2: <a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a> IP in Neuro-2a cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET7106-74" style="font-weight: bold;text-decoration: underline;">ET7106-74</a> in Neuro-2a cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    BDKRB2 was immunoprecipitated from 0.2 mg Neuro-2a cell lysate with ET7106-74 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7106-74 at 1/2,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Neuro-2a cell lysate (input)
    Lane 2: ET7106-74 IP in Neuro-2a cell lysate
    Lane 3: Rabbit IgG instead of ET7106-74 in Neuro-2a cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 seconds; ECL: K1801

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