Endo G Recombinant Rabbit Monoclonal Antibody [JU31-30]
Catalog# ET7106-77
Endo G Recombinant Rabbit Monoclonal Antibody [JU31-30]
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WB
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
Endo G Recombinant Rabbit Monoclonal Antibody [JU31-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Endo G aa 200-297 / 297.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell
Target Molecular Weight
36 kDa
Positive Control
MCF-7, PC-3M, SH-SY5Y, mouse heart tissue lysate.
Conjugation
unconjugated
Clone Number
JU31-30
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
Target
Function
Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria. This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown. In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities. ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. ENDOG exists in the mitochondrial intermembrane space, but not in the matrix where mtDNA replication occurs. This enzyme provides an important nicking function for mitochondrial DNA specifically cleaving DNA at GC tracts. Human ENDOG maps to chromosome 9q34.11.
Background References
1. Gerhard DS et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res 14:2121-2127 (2004).
2. Studencka M et al. Senoptosis: non-lethal DNA cleavage as a route to deep senescence. Oncotarget 8(19):30656-30671 (2017).
Sequence Similarity
Belongs to the DNA/RNA non-specific endonuclease family.
Subcellular Location
Mitochondrion. Nucleus.
Synonyms
EndoG antibody
Endonuclease G antibody
Endonuclease G mitochondrial antibody
EndonucleaseG antibody
FLJ27463 antibody
Mitochondrial endonuclease G antibody
NUCG_HUMAN antibody
Images
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ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised
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Western blot analysis of Endo G on different lysates with Rabbit anti-Endo G antibody (ET7106-77) at 1/1,000 dilution.
Lane 1: Mouse heart tissue lysate
Exposure time: 3 minutes 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7106-77, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32.6 kDa
Observed band size: 36 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"