RPS19 Recombinant Rabbit Monoclonal Antibody [JU33-43]
Catalog# ET7106-94
RPS19 Recombinant Rabbit Monoclonal Antibody [JU33-43]
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WB
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IHC-P
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IP
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IF-Cell
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FC
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
RPS19 Recombinant Rabbit Monoclonal Antibody [JU33-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human RPS19 aa 96-145 / 145.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, IP, IF-Cell, FC
Molecular Weight
Predicted band size: 16 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, 293T cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue, HeLa, NIH/3T3.
Conjugation
unconjugated
Clone Number
JU33-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:3,000
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IP
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1:10-1:50
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Ribosomal subunits are synthesized in the nucleus and mature 40S and 60S subunits are exported stoichiometrically into the cytoplasm. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. Ribosomal proteins have the ability to pass through the nu-clear envelope in the native state, making them the largest of the structures accommodated by the nuclear pore complexes. The nuclear export of ribosomal subunits is a unidirectional, saturable and energy-dependent process. Ribosomal Protein S19 (RPS19) is a 145 amino acids protein expressed in various human adult tissues, including bone marrow, peripheral blood, spleen, liver and nonhematopoietic tissues. RPS19 expression decreases during terminal erythroid differentiation; a deficiency of RPS19 blocks proliferation of immature erythroid progenitor cells altogether. Muta-tions in the RPS19 gene are linked with Diamond-Blackfan anemia (DBA), a congenital, hypo-plastic, red cell aplasia that occasionally presents with physical anomalies.
Background References
1. Flygare J et al. Human RPS19, the gene mutated in Diamond-Blackfan anemia, encodes a ribosomal protein required for the maturation of 40S ribosomal subunits. Blood 109:980-986 (2007).
2. Vladimirov S N et al. Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry. Eur J Biochem 239:144-149 (1996).
Sequence Similarity
Belongs to the eukaryotic ribosomal protein eS19 family.
Tissue Specificity
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Subcellular Location
Cytosol. Nucleus.
Synonyms
40S ribosomal protein S19 antibody
DBA antibody
DBA1 antibody
HGNC:10402 antibody
Ribosomal protein S19 antibody
rps19 antibody
RS19_HUMAN antibody
S19 antibody
Images
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Western blot analysis of RPS19 on different lysates with Rabbit anti-RPS19 antibody (ET7106-94) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-94) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling RPS19 with Rabbit anti-RPS19 antibody (ET7106-94) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RPS19 antibody (ET7106-94) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling RPS19 with Rabbit anti-RPS19 antibody (ET7106-94) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RPS19 antibody (ET7106-94) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RPS19 antibody (ET7106-94) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-94) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RPS19 antibody (ET7106-94) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-94) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-RPS19 antibody (ET7106-94) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-94) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-RPS19 antibody (ET7106-94) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-94) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling RPS19.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET7106-94, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling RPS19.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET7106-94, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"