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Versican Recombinant Rabbit Monoclonal Antibody [JB42-32]

Usd: 385

Specification

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Catalog# ET7107-09

Versican Recombinant Rabbit Monoclonal Antibody [JB42-32]

Application

  • IHC-P

  • WB

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Versican Recombinant Rabbit Monoclonal Antibody [JB42-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Versican aa 3,160-3,396 / 3,396.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, WB, IF-Tissue

Molecular Weight

Predicted band size: 373 kDa

Positive Control

Hela cell lysates, human spleen tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

JB42-32

RRID

AB_2938780

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:50-1:1,000

  • WB

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a lectin-like sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length and degree of modification by glycosaminoglycan chains. Accumulation around smooth muscle cells in lesions of atherosclerosis, suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels. The human verisican gene maps to chromosome 5q14.3.

Background References

1. Miyamoto T et al. Identification of a novel splice site mutation of the CSPG2 gene in a Japanese family with Wagner syndrome. Invest Ophthalmol Vis Sci 46:2726-2735 (2005).

2. Kloeckener-Gruissem B et al. Novel VCAN mutations and evidence for unbalanced alternative splicing in the pathogenesis of Wagner syndrome. Eur J Hum Genet 21:352-356 (2013).

Sequence Similarity

Belongs to the aggrecan/versican proteoglycan family.

Tissue Specificity

Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2: Restricted to normal brain and gliomas. Isoform V3: Found in all these tissues except medulloblastomas.

Post-translational Modification

Phosphorylated by FAM20C in the extracellular medium.

Subcellular Location

Extracellular matrix. Secreted.

UNIPROT

SwissProt: P13611 Human

SwissProt: Q62059 Mouse

Synonyms

Chondroitin sulfate proteoglycan 2 antibody

Chondroitin sulfate proteoglycan core protein 2 antibody

Chondroitin sulfate proteoglycan core protein, cartilage antibody

CSPG2 antibody

CSPG2_HUMAN antibody

ERVR antibody

GHAP antibody

Glial hyaluronate binding protein antibody

Glial hyaluronate-binding protein antibody

Large fibroblast proteoglycan antibody

Expand

Images

  • Western blot analysis of Versican on different lysates with Rabbit anti-Versican antibody (<a href="/products/ET7107-09" style="font-weight: bold;text-decoration: underline;">ET7107-09</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Mouse hippocampus tissue lysate<br />Lane 4: Rat brain tissue lysate<br /><br />Lysates/proteins at 20(cell lysate)/40(tissue lysate）µg/Lane.<br /><br />Predicted band size: 370 kDa<br />Observed band size: 370 kDa<br /><br />Exposure time: 3 minutes<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-09" style="font-weight: bold;text-decoration: underline;">ET7107-09</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Versican on different lysates with Rabbit anti-Versican antibody (ET7107-09) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Mouse hippocampus tissue lysate
    Lane 4: Rat brain tissue lysate

    Lysates/proteins at 20(cell lysate)/40(tissue lysate）µg/Lane.

    Predicted band size: 370 kDa
    Observed band size: 370 kDa

    Exposure time: 3 minutes

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-09) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Versican antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-09" style="font-weight: bold;text-decoration: underline;">ET7107-09</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Versican antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Versican antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-09" style="font-weight: bold;text-decoration: underline;">ET7107-09</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Versican antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Versican antibody (<a href="/products/ET7107-09" style="font-weight: bold;text-decoration: underline;">ET7107-09</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-09" style="font-weight: bold;text-decoration: underline;">ET7107-09</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Versican antibody (ET7107-09) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation