LRG1 Recombinant Rabbit Monoclonal Antibody [JB34-31]
Catalog# ET7107-11
LRG1 Recombinant Rabbit Monoclonal Antibody [JB34-31]
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WB
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IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
LRG1 Recombinant Rabbit Monoclonal Antibody [JB34-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human LRG1 aa 261-310 / 347.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 38 kDa
Positive Control
Human serum lysates, human kidney tissue.
Conjugation
unconjugated
Clone Number
JB34-31
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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IP
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1:10-1:50
Target
Function
Leucine-rich alpha-2-glycoprotein 1 is a protein which in humans is encoded by the gene LRG1. The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation. LRG1 has been shown to be involved in promoting neovascularization (new blood vessel growth) through causing a switch in transforming growth factor beta (TGFbeta) signaling in endothelial cells. LRG1 binds to the accessory receptor endoglin and promotes signaling via the ALK1-Smad1/5/8 pathway.
Background References
1. Takahashi N et al. Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum. Proc Natl Acad Sci USA 82:1906-1910 (1985).
2. Liu T et al. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J Proteome Res 4:2070-2080 (2005).
Tissue Specificity
Plasma.
Subcellular Location
Secreted.
UNIPROT
Synonyms
1300008B03Rik antibody
2310031E04Rik antibody
A2GL antibody
A2GL_HUMAN antibody
HMFT1766 antibody
Leucine rich alpha 2 glycoprotein antibody
Leucine-rich alpha-2-glycoprotein 1 antibody
Leucine-rich alpha-2-glycoprotein antibody
Leucine-rich alpha-2-glycoprotein precursor antibody
LRG antibody
ExpandImages
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Western blot analysis of LRG1 on human serum lysates with Rabbit anti-LRG1 antibody (ET7107-11) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 43 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-11) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LRG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"