Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]
Catalog# ET7107-19
Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]
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WB
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IHC-P
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Mast Cell Chymase aa 198-247 / 247.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 27 kDa
Positive Control
Mouse spleen tissue lysate, human lymph nodes tissue, human skin tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
JB74-32
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:200-1:20,000
Target
Function
Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast Cell Chymase, also known as CMA1 or MCT1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19 amino acid signal peptide, an acidic 2 amino acid propeptide and a 226 amino acid catalytic domain. Mast Cell Chymase is a chymotryptic serine proteinase which is a member of the peptidase family S1. Expressed in mast cells, Mast Cell Chymase is associated with the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function. Mast Cell Protease 1, also designated Mcp-1 or Mcpt1, is a rodent specific beta-chymase. The mouse and rat Mast Cell Protease 1 proteins share 76% sequence identity at the amino acid level.
Background References
1. Schechter N M et al. Determination of the primary structures of human skin chymase and cathepsin G from cutaneous mast cells of urticaria pigmentosa lesions. J Immunol 152:4062-4069 (1994).
2. Caughey G H et al. Structure, chromosomal assignment, and deduced amino acid sequence of a human gene for mast cell chymase. J Biol Chem 266:12956-12963 (1991).
Sequence Similarity
Belongs to the peptidase S1 family. Granzyme subfamily.
Tissue Specificity
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
Subcellular Location
Secreted, Cytoplasmic granule.
Synonyms
Alpha-chymase antibody
Chymase 1 antibody
Chymase 1 mast cell antibody
chymase 1 preproprotein transcript E antibody
chymase 1 preproprotein transcript I antibody
Chymase antibody
Chymase, heart antibody
Chymase, mast cell antibody
CMA1 antibody
CMA1_HUMAN antibody
ExpandImages
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Western blot analysis of Mast Cell Chymase on mouse spleen tissue lysates with Rabbit anti-Mast Cell Chymase antibody (ET7107-19) at 1:1000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7107-19, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 27.3 kDa
Observed band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Mast Cell Chymase antibody (ET7107-19) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"