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Dcp1a Recombinant Rabbit Monoclonal Antibody [JB51-34]

Usd: 385

Specification

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Catalog# ET7107-26

Dcp1a Recombinant Rabbit Monoclonal Antibody [JB51-34]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Dcp1a Recombinant Rabbit Monoclonal Antibody [JB51-34]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Dcp1a aa 460-582 / 582.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 63 kDa

Positive Control

293T cell lysate, MOLT-4 cell lysate, HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, human liver tissue, human pancreas tissue, human esophageal cancer tissue, rat heart issue, rat lung issue.

Conjugation

unconjugated

Clone Number

JB51-34

RRID

AB_3070739

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

mRNA-decapping enzyme 1A is a protein that in humans is encoded by the DCP1A gene. Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. DCP1A has been shown to interact with DCP2 and UPF1. It has also been shown to colocalize with GW182, and other markers of P-body. Human DCP1A is heterotrimeric,[8] and makes contacts with the scaffold EDC3/4. The Arabidopsis thaliana homolog interacts with the plant specific type XI myosin motor protein.

Background References

1. Lykke-Andersen J. Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay. Mol Cell Biol 22:8114-8121 (2002).

2. Bai R-Y et al. SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling. Nat Cell Biol 4:181-190 (2002).

Sequence Similarity

Belongs to the DCP1 family.

Tissue Specificity

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

Subcellular Location

Cytoplasm, P-body, Nucleus.

UNIPROT

SwissProt: Q9NPI6 Human

SwissProt: Q91YD3 Mouse

Entrez Gene: 361109 Rat

Synonyms

DCP1 decapping enzyme homolog A antibody

Dcp1a antibody

DCP1A_HUMAN antibody

Decapping enzyme hDcp1a antibody

Decapping mRNA 1A antibody

HSA275986 antibody

mRNA decapping enzyme 1A antibody

mRNA-decapping enzyme 1A antibody

Nbla00360 antibody

Putative protein product of Nbla00360 antibody

Expand

Images

  • Western blot analysis of Dcp1a on different lysates with Rabbit anti-Dcp1a antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: MOLT-4 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: Mouse brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Dcp1a on different lysates with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/2,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: MOLT-4 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: Mouse brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Dcp1a antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Dcp1a antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue with Rabbit anti-Dcp1a antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat heart issue with Rabbit anti-Dcp1a antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat heart issue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung issue with Rabbit anti-Dcp1a antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-26" style="font-weight: bold;text-decoration: underline;">ET7107-26</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung issue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation