DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
Catalog# ET7107-41
DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
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WB
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DDAH2 aa 1-125 / 285.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 30 kDa
Positive Control
Rat heart tissue lysate, mouse heart tissue lysate, mouse lung tissue lysate, human lung tissue lysate.
Conjugation
unconjugated
Clone Number
JB59-33
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IP
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1:10-:50
Target
Function
DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.
Background References
1. Leiper J M et al. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 343:209-214 (1999).
2. Cillero-Pastor B et al. Dimethylarginine dimethylaminohydrolase 2, a newly identified mitochondrial protein modulating nitric oxide synthesis in normal human chondrocytes. Arthritis Rheum 64:204-212 (2012).
Sequence Similarity
Belongs to the DDAH family.
Tissue Specificity
Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
Subcellular Location
Cytoplasm. Mitochondrion.
Synonyms
DDAH antibody
DDAH II antibody
DDAH-2 antibody
DDAH2 antibody
DDAH2_HUMAN antibody
DDAHII antibody
Dimethylargininase 2 antibody
Dimethylargininase-2 antibody
Dimethylarginine dimethylaminohydrolase 2 antibody
Dimethylarginine dimethylaminohydrolase II antibody
ExpandImages
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Western blot analysis of DDAH2 on different lysates with Rabbit anti-DDAH2 antibody (ET7107-41) at 1/2,000 dilution.
Lane 1: Rat heart tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-41) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"