PU.1/Spi1 Recombinant Rabbit Monoclonal Antibody [JB72-35]
Catalog# ET7107-49
PU.1/Spi1 Recombinant Rabbit Monoclonal Antibody [JB72-35]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
PU.1/Spi1 Recombinant Rabbit Monoclonal Antibody [JB72-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PU1 / Spi1 aa 1-50 / 270.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
THP-1 cell lysates, human spleen tissue, human tonsil tissue, K562.
Conjugation
unconjugated
Clone Number
JB72-35
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found on enhancers of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. The ETS domain is the DNA-binding module of PU.1 and other ETS-family transcription factors.
Background References
1. Basso K et al. BCL6 positively regulates AID and germinal center gene expression via repression of miR-155. J Exp Med 209:2455-2465 (2012).
2. Rao S et al. SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1. J Biol Chem 274:11115-11124 (1999).
Sequence Similarity
Belongs to the ETS family.
Subcellular Location
Cytosol.
UNIPROT
Synonyms
transcription factor spi1 antibody
31 kDa Transforming Protein antibody
31 kDa-transforming protein antibody
cb1086 antibody
Hematopoietic transcription factor PU.1 antibody
OF antibody
oncogene spi1 antibody
PU.1 antibody
SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody
SFPI1 antibody
ExpandImages
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Western blot analysis of PU.1/Spi1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PU.1/Spi1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of PU.1/Spi1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"