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MCM3 Recombinant Rabbit Monoclonal Antibody [JB99-35]

Usd: 385

Specification

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Catalog# ET7107-66

MCM3 Recombinant Rabbit Monoclonal Antibody [JB99-35]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MCM3 Recombinant Rabbit Monoclonal Antibody [JB99-35]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MCM3 aa 1-50 / 808.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 91 kDa

Positive Control

Mouse thymus tissue lysate, NIH-3T3 cell lysates, Hela cell lysates, 293T, HepG2, SH-SY-5Y, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562.

Conjugation

unconjugated

Clone Number

JB99-35

RRID

AB_3070775

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.

Background References

1. Cortez D et al. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc Natl Acad Sci USA 101:10078-10083 (2004).

2. Tsuji T et al. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell 17:4459-4472 (2006).

Sequence Similarity

Belongs to the MCM family.

Post-translational Modification

Acetylated by MCM3AP.; O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location

Chromosome, Nucleus.

UNIPROT

SwissProt: P25205 Human

SwissProt: P25206 Mouse

SwissProt: D3ZFP4 Rat

Synonyms

Cervical cancer proto oncogene 5 antibody

DNA polymerase alpha holoenzyme associated P1 antibody

DNA polymerase alpha holoenzyme associated protein P1 antibody

DNA polymerase alpha holoenzyme-associated protein P1 antibody

DNA replication factor MCM3 antibody

DNA replication licensing factor mcm3 antibody

HCC 5 antibody

HCC5 antibody

hRlf beta subunit antibody

Human cervical cancer proto oncogene 5 antibody

Expand

Images

  • Western blot analysis of MCM3 on HeLa (Human cervical adenocarcinoma cell) lysates with Rabbit anti-MCM3 antibody (<a href="/products/ET7107-66" style="font-weight: bold;text-decoration: underline;">ET7107-66</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7107-66" style="font-weight: bold;text-decoration: underline;">ET7107-66</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 91kDa<br />Observed band size: 91kDa

    Western blot analysis of MCM3 on HeLa (Human cervical adenocarcinoma cell) lysates with Rabbit anti-MCM3 antibody (ET7107-66) at 1/1,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7107-66, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 91kDa
    Observed band size: 91kDa

  • ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation