MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]
Catalog# ET7107-67
MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]
-
WB
-
IF-Cell
-
FC
-
Human
-
unconjugated
Overview
Product Name
MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MYBBP1A aa 1,051-1,100 / 1,328.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 149 kDa
Positive Control
A549 cell lysates, HeLa.
Conjugation
unconjugated
Clone Number
JB40-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
-
IF-Cell
-
1:100
-
FC
-
1:1,000
Target
Function
May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter.
Background References
1. Scherl A et al. Functional proteomic analysis of human nucleolus. Mol Biol Cell 13:4100-4109 (2002).
2. Cavellan E et al. The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription. J Biol Chem 281:16264-16271 (2006).
Sequence Similarity
Belongs to the MYBBP1A family.
Post-translational Modification
Citrullinated by PADI4.
Subcellular Location
Cytoplasm, Nucleus, nucleolus.
UNIPROT
Synonyms
AL024407 antibody
AU019902 antibody
cb486 antibody
FLJ37886 antibody
MBB1A_HUMAN antibody
MYB binding protein (P160) 1a antibody
myb binding protein (P160) 1a like antibody
Myb-binding protein 1A antibody
Mybbp1a antibody
nuclear protein P160 antibody
ExpandImages
-
Western blot analysis of MYBBP1A on A549 cell lysate using anti-MYBBP1A antibody at 1/500 dilution.
-
Immunocytochemistry analysis of HeLa cells labeling MYBBP1A with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling MYBBP1A.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-67, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"