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MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]

Usd: 385

Specification

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Catalog# ET7107-67

MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

MYBBP1A Recombinant Rabbit Monoclonal Antibody [JB40-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MYBBP1A aa 1,051-1,100 / 1,328.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 149 kDa

Positive Control

A549 cell lysates, HeLa.

Conjugation

unconjugated

Clone Number

JB40-09

RRID

AB_3070776

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter.

Background References

1. Scherl A et al. Functional proteomic analysis of human nucleolus. Mol Biol Cell 13:4100-4109 (2002).

2. Cavellan E et al. The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription. J Biol Chem 281:16264-16271 (2006).

Sequence Similarity

Belongs to the MYBBP1A family.

Post-translational Modification

Citrullinated by PADI4.

Subcellular Location

Cytoplasm, Nucleus, nucleolus.

UNIPROT

SwissProt: Q9BQG0 Human

Synonyms

AL024407 antibody

AU019902 antibody

cb486 antibody

FLJ37886 antibody

MBB1A_HUMAN antibody

MYB binding protein (P160) 1a antibody

myb binding protein (P160) 1a like antibody

Myb-binding protein 1A antibody

Mybbp1a antibody

nuclear protein P160 antibody

Expand

Images

  • Western blot analysis of MYBBP1A on Jurkat (Human T-lymphoblastic cells) lysates with Rabbit anti-MYBBP1A antibody (<a href="/products/ET7107-67" style="font-weight: bold;text-decoration: underline;">ET7107-67</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7107-67" style="font-weight: bold;text-decoration: underline;">ET7107-67</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 148.9 kDa<br />Observed band size: 148 kDa

    Western blot analysis of MYBBP1A on Jurkat (Human T-lymphoblastic cells) lysates with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/1,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7107-67, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 148.9 kDa
    Observed band size: 148 kDa

  • Immunocytochemistry analysis of HeLa cells labeling MYBBP1A with Rabbit anti-MYBBP1A antibody (<a href="/products/ET7107-67" style="font-weight: bold;text-decoration: underline;">ET7107-67</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYBBP1A antibody (<a href="/products/ET7107-67" style="font-weight: bold;text-decoration: underline;">ET7107-67</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling MYBBP1A with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MYBBP1A antibody (ET7107-67) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HeLa cells labeling MYBBP1A.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET7107-67" style="font-weight: bold;text-decoration: underline;">ET7107-67</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling MYBBP1A.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-67, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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